Difference between revisions of "Part:BBa K566003"
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Is a monomeric protein generated on the basis of GFP-like protein from jellyfish <i> Aequorea victoria </i>. It possesses bright fluorescence with exitation/emission maxima at 434 and 477 nm, respectively. It has an extinction coefficient of 26,000 M-1 cm-1, which means how strongly a chemical species absorbs light at a given wavelength. It has low absortion and low quantum yield (40%). The fluorescence quantum yield gives the efficiency of the fluorescence process. It is defined as the ratio of the number of photons emitted to the number of photons absorbed. | Is a monomeric protein generated on the basis of GFP-like protein from jellyfish <i> Aequorea victoria </i>. It possesses bright fluorescence with exitation/emission maxima at 434 and 477 nm, respectively. It has an extinction coefficient of 26,000 M-1 cm-1, which means how strongly a chemical species absorbs light at a given wavelength. It has low absortion and low quantum yield (40%). The fluorescence quantum yield gives the efficiency of the fluorescence process. It is defined as the ratio of the number of photons emitted to the number of photons absorbed. | ||
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Revision as of 18:03, 28 September 2011
CFP optimized for E. coli
Cyan fluorescent protein optimized from part BBa_E0022 with preferential codon usage for improved expression in E. coli.
Usage and Biology
Is a monomeric protein generated on the basis of GFP-like protein from jellyfish Aequorea victoria . It possesses bright fluorescence with exitation/emission maxima at 434 and 477 nm, respectively. It has an extinction coefficient of 26,000 M-1 cm-1, which means how strongly a chemical species absorbs light at a given wavelength. It has low absortion and low quantum yield (40%). The fluorescence quantum yield gives the efficiency of the fluorescence process. It is defined as the ratio of the number of photons emitted to the number of photons absorbed.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 28
- 1000COMPATIBLE WITH RFC[1000]