Difference between revisions of "Part:BBa K592009"

Revision as of 09:24, 22 September 2011

amilCP, blue chromoprotein

This chromoprotein from the coral Acropora millepora, amilCP, naturally exhibits strong color when expressed. The color is blue/purple and is visible to naked eye, thereby requiring no instruments to observe. The strong color is readily observed in both LB or agar culture, in less than 24 hours of incubation. The DNA was provided by Jeffrey Miller at UCLA, and was made BioBrick-compatible by removal of one illegal internal restriction site (EcoRI).

Usage and Biology

This part is useful as a reporter. In the pictures below it's built into BBa_K592015.

iGEM11_Uppsala-Sweden: Expression of chromoproteins. The images above show E coli constitutively expressing amilCP BBa_K592009 (blue), amilGFP BBa_K592010 (yellow) and RFP BBa_E1010 (red). Also see the green chromoprotein cjBlue BBa_K592011.

References

[http://www.ncbi.nlm.nih.gov/pubmed/18648549] Alieva, N. O., et al. 2008. Diversity and evolution of coral fluorescent proteins. PLoS One 3:e2680.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 11: / Line 11: @@
 [[Image:Pellets BYR.jpg|300px]]
-'''iGEM11_Uppsala-Sweden:''' Expression of chromoproteins. The images above show ''E coli'' constitutively expressing amilCP <partinfo>BBa_K592009</partinfo> (blue), amilGFP <partinfo>BBa_K592010</partinfo> (yellow) and RFP <partinfo>BBa_E1010</partinfo> (red).
+'''iGEM11_Uppsala-Sweden:''' Expression of chromoproteins. The images above show ''E coli'' constitutively expressing amilCP <partinfo>BBa_K592009</partinfo> (blue), amilGFP <partinfo>BBa_K592010</partinfo> (yellow) and RFP <partinfo>BBa_E1010</partinfo> (red). Also see the green chromoprotein cjBlue <partinfo>BBa_K592011</partinfo>.
 ===References===