Difference between revisions of "Part:BBa K538204:Experience"

Latest revision as of 10:11, 21 September 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K538204

The developers of this brick used it to characterise how expression of the coding region (BBa_K538004) incorporated in this part influences the cold resistance of E. coli, looking at 1) it's ability to survive freeze/thaw cycles, and 2) whether or not expression of CspC allows E. coli to maintain a higher growth rate at suboptimal temperatures. Literature reports of CspC expression enhancing freeze/thaw cycle survival rate in E. coli have been confirmed. Unfortunately, no significant effect of CspC expression was observed in relation to E. coli 's specific growth rate at suboptimal temperatuers. Refer to the experience page of BBa_K538004 for quantitative information on the characterisation experiments.

User Reviews

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[http://2011.igem.org/Team:Amsterdam Amsterdam 2011]

It's our own brick, but we happily confirm it works as intended!

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 ===Applications of BBa_K538204===
-The developers of this brick used it to characterise how expression of the coding region ([[Part:BBa_K538004|BBa_K538004]]) incorporated in this part influences the cold resistance of ''E. coli'', looking at 1) it's ability to survive freeze/thaw cycles, and 2) whether or not expression of ''CspC'' allows ''E. coli'' to maintain a higher growth rate at suboptimal temperatures. Literature reports ''CspC'' expression enhances freeze/thaw cycle survival rate in ''E. coli'' have been confirmed. Unfortunately, no significant effect of ''CspC'' expression was observed in relation to ''E. coli'' 's specific growth rate at suboptimal temperatuers. Refer to the [[Part:BBa_K538004:Experience|experience]] page of [[Part:BBa_K538004|BBa_K538004]] for quantitative information on the characterisation experiments.
+The developers of this brick used it to characterise how expression of the coding region ([[Part:BBa_K538004|BBa_K538004]]) incorporated in this part influences the cold resistance of ''E. coli'', looking at 1) it's ability to survive freeze/thaw cycles, and 2) whether or not expression of ''CspC'' allows ''E. coli'' to maintain a higher growth rate at suboptimal temperatures. Literature reports of ''CspC'' expression enhancing freeze/thaw cycle survival rate in ''E. coli'' have been confirmed. Unfortunately, no significant effect of ''CspC'' expression was observed in relation to ''E. coli'' 's specific growth rate at suboptimal temperatuers. Refer to the [[Part:BBa_K538004:Experience|experience]] page of [[Part:BBa_K538004|BBa_K538004]] for quantitative information on the characterisation experiments.
 ===User Reviews===