Part:BBa_K538004:Experience

Figure 1. Percentage of surviving cells after 1, 2 and 3 freeze/thaw cycles for E. coli that contains a CspC generator (blue) or an empty control vector (black).

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Applications of BBa_K538004

This brick's effect on E. coli 's cold resistance was characterised by its developers, team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011]. Expressing it via BBa_K538204, they looked into 1) whether or not expression of CspC influences growth rate at suboptimal temperatures, and 2) if E. coli comprising this brick experiences enhanced freeze/thaw cycle survival.

CspC is a cold-induced chaperone, so it's conceivably beneficial to growth rate at low temperatures. However, there are no indications of this in literature, so there were no strong a priori expectations about this brick's influence on growth rate. It has been reported CspC strongly increases E. coli 's ability to survive freeze/thaw cycles.[http://www.springerlink.com/index/QR2Q5722J0T78653.pdf]

Expression of this brick wasn't found to have a significant effect on E. coli 's growthrate at suboptimal temperatures, which is as we expected, but not what we'd hoped for. We did, however, manage to reproduce the cold resistant phenotype described by Uh et al. in a freeze/thaw cycle experiment whose results are displayed in figure 1.

References

Uh et al. Rescue of a Cold-Sensitive Mutant at Low Temperatures by Cold Shock Proteins from Polaribacter irgensii KOPRI 22228 J. Microbiol. 48, 798-802 (2010)

User Reviews

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[http://2011.igem.org/Team:Amsterdam Amsterdam 2011]

It's our own brick, but we happily confirm it works as intended!

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