Difference between revisions of "Part:BBa K660003"
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<partinfo>BBa_K660003 short</partinfo> | <partinfo>BBa_K660003 short</partinfo> | ||
| − | A composite part featuring the iLOV biobrick ( | + | A composite part featuring the iLOV biobrick (https://parts.igem.org/Part:BBa_K660004) with an RBS (https://parts.igem.org/Part:BBa_B0030) and double terminator (https://parts.igem.org/Part:BBa_B1006). |
iLOV is a version of LOV2 that has been altered through site directed mutagenesis and DNA shuffling. iLOV has the same function and uses as LOV2, but contains mutations that caused increased intensity in brightness. | iLOV is a version of LOV2 that has been altered through site directed mutagenesis and DNA shuffling. iLOV has the same function and uses as LOV2, but contains mutations that caused increased intensity in brightness. | ||
Latest revision as of 13:34, 21 September 2011
iLOV + RBS + TT
A composite part featuring the iLOV biobrick (https://parts.igem.org/Part:BBa_K660004) with an RBS (https://parts.igem.org/Part:BBa_B0030) and double terminator (https://parts.igem.org/Part:BBa_B1006).
iLOV is a version of LOV2 that has been altered through site directed mutagenesis and DNA shuffling. iLOV has the same function and uses as LOV2, but contains mutations that caused increased intensity in brightness.
As a reporter, it is advantageous over GFP derived fluorescent proteins for a number of reasons. Firstly, it is small in size so can be used when tagging proteins. It also recovers quickly from photobleaching. Uniquely, it has the ability to function in anoxic conditions.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]