Difference between revisions of "Part:BBa K525515"
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Expressed in ''E. coli'', this BioBrick will enable ''E. coli'' to degrade the endocrine disruptor bisphenol A. The fusion protein works better than a polycistronic expression of the two BioBricks ''bisdA'' (<partinfo>K123000</partinfo>) and ''bisdB'' (<partinfo>K123001</partinfo>).  
 
Expressed in ''E. coli'', this BioBrick will enable ''E. coli'' to degrade the endocrine disruptor bisphenol A. The fusion protein works better than a polycistronic expression of the two BioBricks ''bisdA'' (<partinfo>K123000</partinfo>) and ''bisdB'' (<partinfo>K123001</partinfo>).  
  
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This BioBrick was characterized using <partinfo>K525517</partinfo>.
  
 
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Revision as of 20:30, 15 September 2011

Fusion protein of BisdA and BisdB

Fusion protein of BisdA and BisdB for improved bisphenol A degradation with Escherichia coli


Usage and Biology

Expressed in E. coli, this BioBrick will enable E. coli to degrade the endocrine disruptor bisphenol A. The fusion protein works better than a polycistronic expression of the two BioBricks bisdA (BBa_K123000) and bisdB (BBa_K123001).

This BioBrick was characterized using BBa_K525517.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 603
    Illegal BamHI site found at 1341
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 1594
  • 1000
    COMPATIBLE WITH RFC[1000]