Difference between revisions of "Part:BBa K538000:Design"
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===Design Notes===
 
===Design Notes===
−
This protein has been studied extensively by Ferrer ''et al.''[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2004.04077.x/full], including its functionality in ''E. coli''[http://aem.asm.org/cgi/content/abstract/70/8/4499][http://www.nature.com/nbt/journal/v21/n11/full/nbt1103-1266b.html][http://onlinelibrary.wiley.com/doi/10.1002/pmic.200500031/abstract], and as such its sequence[http://www.ncbi.nlm.nih.gov/nuccore/22266159?from=458&to=751&report=gbwithparts] is publicly available. Pre- and suffixes were added to this as clarified in OpenWetWare's BioBrick standards[http://openwetware.org/wiki/Biobrick_standard] and, as is recommended, the TAA stop codon was replaced with TAATAA. The sequence's conformity with Assembly standard 10[https://parts.igem.org/Help:Assembly_standard_10] was ensured using the EMBOSS recoder[http://bioweb2.pasteur.fr/docs/EMBOSS/recoder.html], by recoding restriction sites of EcoRI, XbaI, SpeI, PstI, NotI, PvuII, XhoI, AvrII, NheI and SapI.
+
This protein has been studied extensively by Ferrer ''et al.''[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2004.04077.x/full], including its functionality in ''E. coli''[http://aem.asm.org/cgi/content/abstract/70/8/4499][http://www.nature.com/nbt/journal/v21/n11/full/nbt1103-1266b.html][http://onlinelibrary.wiley.com/doi/10.1002/pmic.200500031/abstract], and as such its [http://www.ncbi.nlm.nih.gov/nuccore/22266159?from=458&to=751&report=gbwithparts sequence] is publicly available. Pre- and suffixes were added to this as clarified in OpenWetWare's [http://openwetware.org/wiki/Biobrick_standard BioBrick standards] and, as is recommended, the TAA stop codon was replaced with TAATAA. The sequence's conformity with [https://parts.igem.org/Help:Assembly_standard_10 Assembly standard 10] was ensured using the [http://bioweb2.pasteur.fr/docs/EMBOSS/recoder.html EMBOSS recoder], by recoding restriction sites of EcoRI, XbaI, SpeI, PstI, NotI, PvuII, XhoI, AvrII, NheI and SapI.
 +
 
 +
''cpn10'' was used by team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011] to create CryoBricks; cold resistance BioBricks. It encodes ''cochaperonin 10'', a part of the ''cpn10/cpn60'' chaperone system of ''Oleispira antarctica'', and thus needs to be coexpressed with ''cpn60'' ([[Part:BBa_K538001|BBa_K538001]]) to be functional. See also the [[Part:BBa_K538000 | Main Page]], or the team's [http://2011.igem.org/Team:Amsterdam/Project/Description Project Description] for further information.
  
  
 
===Source===
 
===Source===
−
''De novo'' synthesis by GeneArt[http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Cloning/gene-synthesis/GeneArt-Gene-Synthesis.html]
+
''De novo'' synthesis by [http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Cloning/gene-synthesis/GeneArt-Gene-Synthesis.html GeneArt]
  
  
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===Patents===
 
===Patents===
−
# '''Ferrer ''et al.''''' Transgenic organisms with lower growth temperature, ''US Patent Number 7,811,784''
+
# '''Ferrer ''et al.''''' Transgenic organisms with lower growth temperature, ''US Patent Number 7,811,784'' [http://www.google.com/patents/about?id=cGDXAAAAEBAJ (View in Google Patents)]

Revision as of 10:40, 29 July 2011

Cpn10 (O. antarctica)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 52


Design Notes

This protein has been studied extensively by Ferrer et al.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2004.04077.x/full], including its functionality in E. coli[http://aem.asm.org/cgi/content/abstract/70/8/4499][http://www.nature.com/nbt/journal/v21/n11/full/nbt1103-1266b.html][http://onlinelibrary.wiley.com/doi/10.1002/pmic.200500031/abstract], and as such its [http://www.ncbi.nlm.nih.gov/nuccore/22266159?from=458&to=751&report=gbwithparts sequence] is publicly available. Pre- and suffixes were added to this as clarified in OpenWetWare's [http://openwetware.org/wiki/Biobrick_standard BioBrick standards] and, as is recommended, the TAA stop codon was replaced with TAATAA. The sequence's conformity with Assembly standard 10 was ensured using the [http://bioweb2.pasteur.fr/docs/EMBOSS/recoder.html EMBOSS recoder], by recoding restriction sites of EcoRI, XbaI, SpeI, PstI, NotI, PvuII, XhoI, AvrII, NheI and SapI.

cpn10 was used by team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011] to create CryoBricks; cold resistance BioBricks. It encodes cochaperonin 10, a part of the cpn10/cpn60 chaperone system of Oleispira antarctica, and thus needs to be coexpressed with cpn60 (BBa_K538001) to be functional. See also the Main Page, or the team's [http://2011.igem.org/Team:Amsterdam/Project/Description Project Description] for further information.


Source

De novo synthesis by [http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Cloning/gene-synthesis/GeneArt-Gene-Synthesis.html GeneArt]


References

  1. Ferrer et al. Functional consequences of single:double ring transitions in chaperonins: life in the cold, Mol. Microbiol. 53 (1), 167-182 (2004)
  2. Ferrer et al. Expression of a Temperature-Sensitive Esterase in a Novel Chaperone-Based Escherichia coli Strain, App. Env. Microbiol. 70 (8), 4499-4504 (2004)
  3. Ferrer et al. Chaperonins govern growth of Escherichia coli at low temperatures, Nat. Biotech. 21, 1266 - 1267 (2003)
  4. Strocchi, Ferrer, Timmis & Golyshin Low temperature-induced systems failure in Escherichia coli: Insights from rescue by cold-adapted chaperones, Proteomics 6 (1), 193-206 (2005)


Patents

  1. Ferrer et al. Transgenic organisms with lower growth temperature, US Patent Number 7,811,784 [http://www.google.com/patents/about?id=cGDXAAAAEBAJ (View in Google Patents)]