Difference between revisions of "Part:BBa K538000:Design"
Line 6: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
The protein's sequence was published by Ferrer ''et al.'' in 2004[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2004.04077.x/full]. Pre- and suffixes were added to this as clarified in OpenWetWare's BioBrick standards[http://openwetware.org/wiki/Biobrick_standard] and, as is recommended, the TAA stop codon was replaced with TAATAA. The sequence's conformity with Assembly standard 10[https://parts.igem.org/Help:Assembly_standard_10] was ensured using the EMBOSS recoder[http://bioweb2.pasteur.fr/docs/EMBOSS/recoder.html], by recoding restriction sites of EcoRI, XbaI, SpeI, PstI, NotI, PvuII, XhoI, AvrII, NheI and SapI.
+
This protein has been studied extensively by Ferrer ''et al.''[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2004.04077.x/full], including its functionality in ''E. coli''[http://aem.asm.org/cgi/content/abstract/70/8/4499][http://www.nature.com/nbt/journal/v21/n11/full/nbt1103-1266b.html][http://onlinelibrary.wiley.com/doi/10.1002/pmic.200500031/abstract], and as such its sequence[http://www.ncbi.nlm.nih.gov/nuccore/22266159?from=458&to=751&report=gbwithparts] is publicly available. Pre- and suffixes were added to this as clarified in OpenWetWare's BioBrick standards[http://openwetware.org/wiki/Biobrick_standard] and, as is recommended, the TAA stop codon was replaced with TAATAA. The sequence's conformity with Assembly standard 10[https://parts.igem.org/Help:Assembly_standard_10] was ensured using the EMBOSS recoder[http://bioweb2.pasteur.fr/docs/EMBOSS/recoder.html], by recoding restriction sites of EcoRI, XbaI, SpeI, PstI, NotI, PvuII, XhoI, AvrII, NheI and SapI.
  
 
===Source===
 
===Source===
Line 13: Line 13:
  
 
===References===
 
===References===
−
'''Ferrer ''et al.''''' Functional consequences of single:double ring transitions in chaperonins: life in the cold, ''Mol. Microbiol. 53 (1), 167-182 (2004)''
+
# '''Ferrer ''et al.''''' Functional consequences of single:double ring transitions in chaperonins: life in the cold, ''Mol. Microbiol. 53 (1), 167-182 (2004)''
 +
# '''Ferrer ''et al.''''' Expression of a Temperature-Sensitive Esterase in a Novel Chaperone-Based ''Escherichia coli'' Strain, ''App. Env. Microbiol. 70 (8), 4499-4504 (2004)''
 +
# '''Ferrer ''et al.''''' Chaperonins govern growth of ''Escherichia coli'' at low temperatures, ''Nat. Biotech. 21, 1266 - 1267 (2003)''
 +
# '''Strocchi, Ferrer, Timmis & Golyshin''' Low temperature-induced systems failure in ''Escherichia coli'': Insights from rescue by cold-adapted chaperones, ''Proteomics 6 (1), 193-206 (2005)''

Revision as of 09:24, 29 July 2011

Cpn10 (O. antarctica)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 52


Design Notes

This protein has been studied extensively by Ferrer et al.[http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2004.04077.x/full], including its functionality in E. coli[http://aem.asm.org/cgi/content/abstract/70/8/4499][http://www.nature.com/nbt/journal/v21/n11/full/nbt1103-1266b.html][http://onlinelibrary.wiley.com/doi/10.1002/pmic.200500031/abstract], and as such its sequence[http://www.ncbi.nlm.nih.gov/nuccore/22266159?from=458&to=751&report=gbwithparts] is publicly available. Pre- and suffixes were added to this as clarified in OpenWetWare's BioBrick standards[http://openwetware.org/wiki/Biobrick_standard] and, as is recommended, the TAA stop codon was replaced with TAATAA. The sequence's conformity with Assembly standard 10[1] was ensured using the EMBOSS recoder[http://bioweb2.pasteur.fr/docs/EMBOSS/recoder.html], by recoding restriction sites of EcoRI, XbaI, SpeI, PstI, NotI, PvuII, XhoI, AvrII, NheI and SapI.

Source

De novo synthesis by GeneArt[http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Cloning/gene-synthesis/GeneArt-Gene-Synthesis.html]

References

  1. Ferrer et al. Functional consequences of single:double ring transitions in chaperonins: life in the cold, Mol. Microbiol. 53 (1), 167-182 (2004)
  2. Ferrer et al. Expression of a Temperature-Sensitive Esterase in a Novel Chaperone-Based Escherichia coli Strain, App. Env. Microbiol. 70 (8), 4499-4504 (2004)
  3. Ferrer et al. Chaperonins govern growth of Escherichia coli at low temperatures, Nat. Biotech. 21, 1266 - 1267 (2003)
  4. Strocchi, Ferrer, Timmis & Golyshin Low temperature-induced systems failure in Escherichia coli: Insights from rescue by cold-adapted chaperones, Proteomics 6 (1), 193-206 (2005)