Difference between revisions of "Part:BBa K538000:Design"

 
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<partinfo>BBa_K538000 short</partinfo>
 
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===Design Notes===
 
===Design Notes===
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The protein's sequence[1] was published by Ferrer ''et al.'' in 2004. Pre- and suffixes were added to this as clarified in OpenWetWare's BioBrick standards[2] and, as is recommended, the TAA stop codon was replaced with TAATAA. The sequence's conformity with Assembly standard 10[3] was confirmed using the EMBOSS recoder[4], by recoding restriction sites of EcoRI, XbaI, SpeI, PstI, NotI, PvuII, XhoI, AvrII, NheI and SapI.
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The protein's sequence was published by Ferrer ''et al.'' in 2004[http://www.ncbi.nlm.nih.gov/nuccore/22266159?from=458&to=751&report=gbwithparts]. Pre- and suffixes were added to this as clarified in OpenWetWare's BioBrick standards[http://openwetware.org/wiki/Biobrick_standard] and, as is recommended, the TAA stop codon was replaced with TAATAA. The sequence's conformity with Assembly standard 10[https://parts.igem.org/Help:Assembly_standard_10] was ensured using the EMBOSS recoder[http://bioweb2.pasteur.fr/docs/EMBOSS/recoder.html], by recoding restriction sites of EcoRI, XbaI, SpeI, PstI, NotI, PvuII, XhoI, AvrII, NheI and SapI.
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1 - http://www.ncbi.nlm.nih.gov/nuccore/22266159?from=458&to=751&report=gbwithparts
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2 - http://openwetware.org/wiki/Biobrick_standard
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3 - https://parts.igem.org/Help:Assembly_standard_10
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4 - http://bioweb2.pasteur.fr/docs/EMBOSS/recoder.html
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===Source===
 
===Source===

Revision as of 12:19, 28 July 2011

Cpn10 (O. antarctica)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 52


Design Notes

The protein's sequence was published by Ferrer et al. in 2004[http://www.ncbi.nlm.nih.gov/nuccore/22266159?from=458&to=751&report=gbwithparts]. Pre- and suffixes were added to this as clarified in OpenWetWare's BioBrick standards[http://openwetware.org/wiki/Biobrick_standard] and, as is recommended, the TAA stop codon was replaced with TAATAA. The sequence's conformity with Assembly standard 10[1] was ensured using the EMBOSS recoder[http://bioweb2.pasteur.fr/docs/EMBOSS/recoder.html], by recoding restriction sites of EcoRI, XbaI, SpeI, PstI, NotI, PvuII, XhoI, AvrII, NheI and SapI.

Source

De novo synthesis

References