Difference between revisions of "Part:BBa K325909:ArabinosetoLight"
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− | [[Image:BBa_K325909Aracurve.png|thumb|569px|center|'''Figure 1 - Light output of <partinfo> | + | [[Image:BBa_K325909Aracurve.png|thumb|569px|center|'''Figure 1 - Light output of <partinfo>K325909</partinfo> as a function of Arabinose concentration in the media. The values correspond to the peak intensity within 5 hours of adding Arabinose to the media. Data points and error bars correspond to the mean and the standard deviation of 3 time repeats. ''']] |
− | [[Image:BBa_K325909timecourse.png|thumb|569px|center|'''Figure 2 - Evolution of luminescence with time at different Arabinose concentrations for <partinfo> | + | [[Image:BBa_K325909timecourse.png|thumb|569px|center|'''Figure 2 - Evolution of luminescence with time at different Arabinose concentrations for <partinfo>K325909</partinfo>. Measurements were taken every 30 minutes. Data points and error bars correspond to the mean and standard deviation of 3 time repeats. ''']] |
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</div>Measured by the [http://2010.igem.org/Team:Cambridge Cambridge iGEM team 2010] | </div>Measured by the [http://2010.igem.org/Team:Cambridge Cambridge iGEM team 2010] | ||
<div style="padding: 00px; width: 680px"> | <div style="padding: 00px; width: 680px"> | ||
'''Compatibility'''<br> | '''Compatibility'''<br> | ||
− | [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=cell ''Chassis:''] Device has been shown to work in ''Top 10 (Invitrogen)''<br> | + | [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=cell ''Chassis:''] Device has been shown to work in ''Top 10 (Invitrogen)'', [http://www.ecoliwiki.net/colipedia/index.php/BW25113 BW25113 DELTA H-NS::kan] and H-NS mutant JM 230 H-NS -205::tn10.<br> |
[[Plasmid backbones|''Plasmids:'']] Device has been shown to work on ''<partinfo>pSB1C3</partinfo>'' <br> | [[Plasmid backbones|''Plasmids:'']] Device has been shown to work on ''<partinfo>pSB1C3</partinfo>'' <br> | ||
Latest revision as of 21:21, 27 October 2010
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Description
This page describes the light output of BBa K325909 as a fucntion of Arabinose concentration in the media. The characterisation has been made in different chassis. TOP 10 cells as well as 2 mutants in which expression of H-NS was repressed.
Data
Data | Notes | Date Uploaded |
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Media:BBa_K325909ArabinosetoLight.xls | Raw data from experiment | 21/10/2010 |
Compatibility
Chassis: Device has been shown to work in Top 10 (Invitrogen), [http://www.ecoliwiki.net/colipedia/index.php/BW25113 BW25113 DELTA H-NS::kan] and H-NS mutant JM 230 H-NS -205::tn10.
Plasmids: Device has been shown to work on pSB1C3
References
[http://www.jstor.org/stable/4449975 [1]:] J. Slock, (1995) Transformation Experiments Using Bioluminescence Genes of Vibrio fischeri,The American Biology Teacher, 57, 225-227.
[http://www.annualreviews.org/doi/pdf/10.1146/annurev.mi.42.100188.001055 [2]:] E.A. Meighen (1988) Enzymes and genes from the lux operons of bioluminescent bacteria, Annual Reviews in Microbiology 42, 151-176. [http://www.annualreviews.org/doi/pdf/10.1146/annurev.ge.28.120194.001001 [3]:] E.A. Meighen, (1994) Genetics of bacterial bioluminescence, Annual Reviews of Genetics, 28, 117-139.