Difference between revisions of "Part:BBa K395602"
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<partinfo>BBa_K395602 short</partinfo> | <partinfo>BBa_K395602 short</partinfo> | ||
| − | + | BBa_K395602 produce MpAAT1 enzyme that catalyzes production of 2-methylbutyl acetate and butyl acetate from 2-methylbutanol and butanol. 2-methylbutyl acetate and butyl acetate has a apple fragrance. | |
| − | + | We designed apple fragrance expression device with MpAAT1<sup>[1]</sup>. Fig. 1 shows the outline of the device. MpAAT1 converts substrate and Acetyl CoA into apple fragrance molecules. Acetyl CoA exists originally in E.coli cell. MpAAT1 converts 2-methyl butanol and butanol into 2-methylbutyl acetate and butyl acetate respectively. | |
| − | [[Image: | + | [[Image:K395602.png|600px|center|thumb|Fig. 1. Apple fragrance expression device.]] |
| − | (1) MpAAT1 + BuOH, (2) No MpAAT1 + BuOAc, (3) No MpAAT1 + BuOH, (4) MpAAT1 + 2-MeBuOH, (5) No MpAAT1 + 2-MeBuOAc, (6) No MpAAT1 + 2-MeBuOH, (7) MpAAT1 - 2-MeBuOH. | + | |
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| + | We performed gas chromatography to confirm the production of the esters, and the results revealed that MpAAT1 successfully converted alcohol and Acetyl CoA into our target esters (Fig. 2). | ||
| + | |||
| + | [[Image:Tokyotech gas chromatograpy.png|600px|center|thumb|Fig. 2. Gas chromatography analysis of apple fragrance expression device.<br>(1) MpAAT1 + BuOH, (2) No MpAAT1 + BuOAc, (3) No MpAAT1 + BuOH, (4) MpAAT1 + 2-MeBuOH, (5) No MpAAT1 + 2-MeBuOAc, (6) No MpAAT1 + 2-MeBuOH, (7) MpAAT1 - 2-MeBuOH.<Br> | ||
| + | This work is done by Toshitaka Matsubara.]] | ||
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| + | (→[http://2010.igem.org/Team:Tokyo_Tech/Project/Apple_Reporter2 more information]) | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K395602 parameters</partinfo> | <partinfo>BBa_K395602 parameters</partinfo> | ||
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| + | ==Functional Parameters: Austin_UTexas== | ||
| + | <html> | ||
| + | <body> | ||
| + | <h3><center>Burden Imposed by this Part:</center></h3> | ||
| + | <figure> | ||
| + | <div class = "center"> | ||
| + | <center><img src = "https://static.igem.org/mediawiki/parts/7/7a/T--Austin_Utexas--high_significant_burden.png" style = "width:250px;height:120px"></center> | ||
| + | </div> | ||
| + | <figcaption><center><b>Burden Value: 20.3 ± 1.9% </b></center></figcaption> | ||
| + | </figure> | ||
| + | <p> Burden is the percent reduction in the growth rate of <i>E. coli</i> cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This part exhibited a significant burden. Users should be aware that BioBricks with a burden of >20-30% may be susceptible to mutating to become less functional or nonfunctional as an evolutionary consequence of this fitness cost. This risk increases as they used for more bacterial cell divisions or in larger cultures. Users should be especially careful when combining multiple burdensome parts, as plasmids with a total burden of >40% are expected to mutate so quickly that they become unclonable. Refer to any one of the | ||
| + | <a href="https://parts.igem.org/Part:BBa_K3174002">BBa_K3174002</a> - <a href="https://parts.igem.org/Part:BBa_K3174007">BBa_K3174007</a> pages for more information on the methods and other conclusions from a large-scale measurement project conducted by the <a href="https://2019.igem.org/Team:Austin_UTexas">2019 Austin_UTexas team.</a></p> | ||
| + | <p>This functional parameter was added by the <a href="https://2020.igem.org/Team:Austin_UTexas/Contribution">2020 Austin_UTexas team.</a></p> | ||
| + | </p> | ||
| + | </body> | ||
| + | </html> | ||
Latest revision as of 00:49, 4 September 2020
Apple fragrance generator
BBa_K395602 produce MpAAT1 enzyme that catalyzes production of 2-methylbutyl acetate and butyl acetate from 2-methylbutanol and butanol. 2-methylbutyl acetate and butyl acetate has a apple fragrance.
We designed apple fragrance expression device with MpAAT1[1]. Fig. 1 shows the outline of the device. MpAAT1 converts substrate and Acetyl CoA into apple fragrance molecules. Acetyl CoA exists originally in E.coli cell. MpAAT1 converts 2-methyl butanol and butanol into 2-methylbutyl acetate and butyl acetate respectively.
We performed gas chromatography to confirm the production of the esters, and the results revealed that MpAAT1 successfully converted alcohol and Acetyl CoA into our target esters (Fig. 2).
(→[http://2010.igem.org/Team:Tokyo_Tech/Project/Apple_Reporter2 more information])
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1765
Illegal NotI site found at 1666 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 195
Illegal BamHI site found at 1635
Illegal XhoI site found at 1675 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 205
Illegal AgeI site found at 1419 - 1000COMPATIBLE WITH RFC[1000]
Functional Parameters
Functional Parameters: Austin_UTexas
Burden Imposed by this Part:
Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This part exhibited a significant burden. Users should be aware that BioBricks with a burden of >20-30% may be susceptible to mutating to become less functional or nonfunctional as an evolutionary consequence of this fitness cost. This risk increases as they used for more bacterial cell divisions or in larger cultures. Users should be especially careful when combining multiple burdensome parts, as plasmids with a total burden of >40% are expected to mutate so quickly that they become unclonable. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.
This functional parameter was added by the 2020 Austin_UTexas team.