Difference between revisions of "Part:BBa K404213"

Latest revision as of 02:43, 28 October 2010

ViralBrick-587KO-Z34C

pCMV_VP1up_NLS_mVenus_(AAV2)-VP13(ViralBrick-587KO-Empty)
BioBrick Nr.	BBa_K404165
RFC standard	RFC 10
Requirement	pSB1C3
Source
Submitted by	[http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010]

The Z34C antibody binding motif including a knockout of the natural HSPG tropism, ready for insertion into the 587 loop

Usage and Biology

The Z34C antibody binding motif is a 34 amino acids long fragment from the a minimized fragment of the Z Domain of Staphylococcal Protein A. It specifically binds to the Fc-Domain of antibodies. If inserted into AAV capsids, it can bind antibodies against a specific antigen, allowing differential targeting without the need for further genetic engineering.

Restriction sites

Restriction site	Enzyme	Recognition sequence	Activity	Temp.
upstream 587	[http://www.neb.com/nebecomm/products/productR3136.asp BamHI-HF]		100;50;10;100	37°C
downstream 587	[http://www.neb.com/nebecomm/products/productR3151.asp PvuII-HF]		0;25;0;100	37°C

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 10
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K404213 short</partinfo>
+{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="left"
+! colspan="2" style="background:#66bbff;"|[https://parts.igem.org/Part:BBa_K404165 pCMV_VP1up_NLS_mVenus_(AAV2)-VP13(ViralBrick-587KO-Empty)]
+|-
+|'''BioBrick Nr.'''
+|[https://parts.igem.org/Part:BBa_K404165 BBa_K404165]
+|-
+|'''RFC standard'''
+|[https://parts.igem.org/Help:Assembly_standard_10 RFC 10]
+|-
+|'''Requirement'''
+|pSB1C3<br>
+|-
+|'''Source'''
+|
+|-
+|'''Submitted by'''
+|[http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010]
+|}
+<br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/>
 <br><b>The Z34C antibody binding motif including a knockout of the natural HSPG tropism, ready for insertion into the 587 loop</b>
@@ Line 7: / Line 28: @@
 ===Usage and Biology===
+<html>
+<div style="width:965px; height:400px; ">
+<div style="float:left; width:460px; height:auto;  margin: 0px 5px 0px 5px; text-align:justify;">
+The Z34C antibody binding motif is a 34 amino acids long fragment from the a minimized fragment of the Z Domain of  <a href=http://www.ncbi.nlm.nih.gov/protein/153106>Staphylococcal Protein A</a>. It specifically binds to the Fc-Domain of antibodies. If inserted into AAV capsids, it can bind antibodies against a specific antigen, allowing differential targeting without the need for further genetic engineering.
+</div>
+<div style="float:right; width:480px; height:auto; "><img src="https://static.igem.org/mediawiki/parts/9/98/Freiburg10_ViralBrick_motif_Z34C.png" width="460"
+height="auto"/></div></div>
+</html>
-The Z34C antibody binding motif is a 34 amino acids long fragment from the a minimized fragment of the Z Domain of  [http://www.ncbi.nlm.nih.gov/protein/153106 Staphylococcal Protein A]. It specifically binds to the Fc-Domain of antibodies. If inserted into AAV capsids, it can bind antibodies against a specific antigen, allowing differential targeting without the need for further genetic engineering.
-[[Image:Freiburg10_ViralBrick_motif_Z34C.png|thumb|center|480px]]
 <!-- -->
 ===Restriction sites===
@@ Line 39: / Line 66: @@
 <tr>
 </table>
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K404213 SequenceAndFeatures</partinfo>