Difference between revisions of "Part:BBa K404213"
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K404213 short</partinfo>
 
<partinfo>BBa_K404213 short</partinfo>
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{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="left"
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! colspan="2" style="background:#66bbff;"|[https://parts.igem.org/Part:BBa_K404165 pCMV_VP1up_NLS_mVenus_(AAV2)-VP13(ViralBrick-587KO-Empty)]
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|-
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|'''BioBrick Nr.'''
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|[https://parts.igem.org/Part:BBa_K404165 BBa_K404165]
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|-
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|'''RFC standard'''
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|[https://parts.igem.org/Help:Assembly_standard_10 RFC 10]
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|-
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|'''Requirement'''
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|pSB1C3<br>
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|-
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|'''Source'''
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|
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|-
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|'''Submitted by'''
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|[http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010]
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|}
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<br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/>
 
<br><b>The Z34C antibody binding motif including a knockout of the natural HSPG tropism, ready for insertion into the 587 loop</b>
 
<br><b>The Z34C antibody binding motif including a knockout of the natural HSPG tropism, ready for insertion into the 587 loop</b>
  
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[[Image:Freiburg10_ViralBrick-logo-453-Z34C.png|thumb|center|480px]]<br>
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[[Image:Freiburg10_ViralBrick-logo-587KO-Z34C.png|thumb|center|480px]]<br>
  
  
 
===Usage and Biology===
 
===Usage and Biology===
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<html>
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<div style="width:965px; height:400px; ">
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<div style="float:left; width:460px; height:auto;  margin: 0px 5px 0px 5px; text-align:justify;">
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The Z34C antibody binding motif is a 34 amino acids long fragment from the a minimized fragment of the Z Domain of  <a href=http://www.ncbi.nlm.nih.gov/protein/153106>Staphylococcal Protein A</a>. It specifically binds to the Fc-Domain of antibodies. If inserted into AAV capsids, it can bind antibodies against a specific antigen, allowing differential targeting without the need for further genetic engineering.
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</div>
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<div style="float:right; width:480px; height:auto; "><img src="https://static.igem.org/mediawiki/parts/9/98/Freiburg10_ViralBrick_motif_Z34C.png" width="460"
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height="auto"/></div></div>
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</html>
  
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The Z34C antibody binding motif is a 34 amino acids long fragment from the a minimized fragment of the Z Domain of  [http://www.ncbi.nlm.nih.gov/protein/153106 Staphylococcal Protein A]. It specifically binds to the Fc-Domain of antibodies. If inserted into AAV capsids, it can bind antibodies against a specific antigen, allowing differential targeting without the need for further genetic engineering.
 
  
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[[Image:Freiburg10_ViralBrick_motif_Z34C.png|thumb|center|480px]]
 
 
<!-- -->
 
<!-- -->
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===Restriction sites===
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<table border="1" style="float:right; width:480px; height:auto;>
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<tr>
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  <th><b>Restriction site</b></th>
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  <th><b>Enzyme</b></th>
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  <th><b>Recognition sequence</b></th>
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  <th><b>Activity</b></th>
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  <th><b>Temp.</b></th>
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</tr>
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<tr>
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  <td>upstream 587</td>
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  <td>[http://www.neb.com/nebecomm/products/productR3136.asp BamHI-HF]</td>
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  <td>[[image:Freiburg10_recognition site BamHI-HF.Gif]]</td>
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  <td>100;50;10;100</td>
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  <td>37°C</td>
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</tr>
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<tr>
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  <td>downstream 587</td>
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  <td>[http://www.neb.com/nebecomm/products/productR3151.asp PvuII-HF]</td>
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  <td>[[image:Freiburg10_recognition site PvuII-HF.Gif]]</td>
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  <td>0;25;0;100</td>
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  <td>37°C
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</tr>
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<tr>
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</table>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K404213 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K404213 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 02:43, 28 October 2010

ViralBrick-587KO-Z34C


pCMV_VP1up_NLS_mVenus_(AAV2)-VP13(ViralBrick-587KO-Empty)
BioBrick Nr. BBa_K404165
RFC standard RFC 10
Requirement pSB1C3
Source
Submitted by [http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010]















The Z34C antibody binding motif including a knockout of the natural HSPG tropism, ready for insertion into the 587 loop

Freiburg10 ViralBrick-logo-587KO-Z34C.png


Usage and Biology

The Z34C antibody binding motif is a 34 amino acids long fragment from the a minimized fragment of the Z Domain of Staphylococcal Protein A. It specifically binds to the Fc-Domain of antibodies. If inserted into AAV capsids, it can bind antibodies against a specific antigen, allowing differential targeting without the need for further genetic engineering.


Restriction sites

Restriction site Enzyme Recognition sequence Activity Temp.
upstream 587 [http://www.neb.com/nebecomm/products/productR3136.asp BamHI-HF] Freiburg10 recognition site BamHI-HF.Gif 100;50;10;100 37°C
downstream 587 [http://www.neb.com/nebecomm/products/productR3151.asp PvuII-HF] Freiburg10 recognition site PvuII-HF.Gif 0;25;0;100 37°C


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 10
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]