Difference between revisions of "Part:BBa K316018"

m
 
(6 intermediate revisions by the same user not shown)
Line 2: Line 2:
 
<partinfo>BBa_K316018 short</partinfo>
 
<partinfo>BBa_K316018 short</partinfo>
  
−
ComE binds to a specific sequence leading to activation of transcription. This is regulating the ComE responsive promoter from the ComCDE operon. This sequence has been previously shown to be sufficient to drive ComE regulated transcription within ''S. pneumoniae'' (''Halfmann et al'').
+
ComE <bbpart>BBa_K316016</bbpart> binds to a specific sequence in a promoter leading to activation of transcription downstream of the binding site. This sequence regulates the ComE responsive promoter in the ComCDE operon. This sequence has been previously shown to be sufficient to drive ComE regulated transcription within ''S. pneumoniae''[http://www.ncbi.nlm.nih.gov/pubmed/17328748].
 +
 
 +
 
 +
[[Image:ComEbs.PNG|center|500px]]
 +
 
 +
'''Figure I.''' ComE binding sites in ''S. pneumonia'' genome. Top - ComAB operon, Middle - ComCDE operon, Bottom - consensus sequence. Source[http://www.ncbi.nlm.nih.gov/pubmed/15126469]
 +
 
 +
For more information about ComCDE system, please check our wiki [http://2010.igem.org/Team:Imperial_College_London/Modules/Signaling]
 +
 
  
  
Line 9: Line 17:
  
 
<!-- -->
 
<!-- -->
−
<span class='h3bb'>Sequence and Features</span>
+
<span class='h3bb'><big>'''Sequence and Features'''</big></span>
 +
 
 +
 
 
<partinfo>BBa_K316018 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K316018 SequenceAndFeatures</partinfo>
  
Line 22: Line 32:
 
<biblio>
 
<biblio>
  
−
#1 ''A new integrative reporter plasmid for Streptococcus pneumoniae. Halfmann et al (2007)''
+
#1 A new integrative reporter plasmid for Streptococcus pneumoniae. Halfmann et al (2007)[http://www.ncbi.nlm.nih.gov/pubmed/17328748]
  
 
</biblio>
 
</biblio>

Latest revision as of 23:53, 27 October 2010

ComE responsive promoter

ComE BBa_K316016 binds to a specific sequence in a promoter leading to activation of transcription downstream of the binding site. This sequence regulates the ComE responsive promoter in the ComCDE operon. This sequence has been previously shown to be sufficient to drive ComE regulated transcription within S. pneumoniae[http://www.ncbi.nlm.nih.gov/pubmed/17328748].


ComEbs.PNG

Figure I. ComE binding sites in S. pneumonia genome. Top - ComAB operon, Middle - ComCDE operon, Bottom - consensus sequence. Source[http://www.ncbi.nlm.nih.gov/pubmed/15126469]

For more information about ComCDE system, please check our wiki [http://2010.igem.org/Team:Imperial_College_London/Modules/Signaling]


Sequence and Features



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



References

<biblio>

  1. 1 A new integrative reporter plasmid for Streptococcus pneumoniae. Halfmann et al (2007)[http://www.ncbi.nlm.nih.gov/pubmed/17328748]

</biblio>