Difference between revisions of "Part:BBa K329055"

 
 
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<partinfo>BBa_K329055 short</partinfo>
 
<partinfo>BBa_K329055 short</partinfo>
  
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This part is composed of a Kanamycine resistance and LacZ cassette between two mutated arms (left and right) of the transposon Tn916 containing each of us one half of the attR lambda recombination site.
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When the both enzyme of the transposon,Int and Xis tn916 are expressed (see <partinfo>BBa_K329035</partinfo> and <partinfo>BBa_K329018</partinfo>)., the both arms link together forming a circular DNA sequence excised from the hosting DNA (plasmid or chromosome). The excision permit to create a functionnal attR lambda recombination site.
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This mutant provide a high efficiency excision characterized (see experience part).
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K329055 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K329055 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 19:23, 5 November 2010

[KanR + LacZ] in between MUT_MUT[Lambda] Tn916 Arms

This part is composed of a Kanamycine resistance and LacZ cassette between two mutated arms (left and right) of the transposon Tn916 containing each of us one half of the attR lambda recombination site. When the both enzyme of the transposon,Int and Xis tn916 are expressed (see BBa_K329035 and BBa_K329018)., the both arms link together forming a circular DNA sequence excised from the hosting DNA (plasmid or chromosome). The excision permit to create a functionnal attR lambda recombination site. This mutant provide a high efficiency excision characterized (see experience part).

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 363
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 382
    Illegal NheI site found at 405
    Illegal SpeI site found at 363
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 363
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 363
  • 1000
    COMPATIBLE WITH RFC[1000]