Difference between revisions of "Part:BBa K329017"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K329017 short</partinfo>
 
<partinfo>BBa_K329017 short</partinfo>
  
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This part is one of our population counters. When IntI1 (<partinfo>BBa_K329014</partinfo>) is expressed, it excises the double terminator flanked by attC sites (<partinfo>BBa_J99001</partinfo>) and triggers the expression of TetA(C) (with a medium RBS, <partinfo>BBa_K329000</partinfo>) because of the constitutive promoter upstream.
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This part is one of our population counters. When IntI1 (<partinfo>BBa_K329014</partinfo>) is expressed, it excises the double terminator flanked by attC sites (<partinfo>BBa_J99001</partinfo>) and triggers the expression of TetA(C) (with a medium RBS, <partinfo>BBa_K329000</partinfo>) and LuxI (<partinfo>BBa_K081008</partinfo>) because of the constitutive promoter upstream.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 17:53, 24 October 2010

Population counter Tetracycline resistant - PmS-MTet-LuxI

This part is one of our population counters. When IntI1 (BBa_K329014) is expressed, it excises the double terminator flanked by attC sites (BBa_J99001) and triggers the expression of TetA(C) (with a medium RBS, BBa_K329000) and LuxI (BBa_K081008) because of the constitutive promoter upstream.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 6
    Illegal NheI site found at 29
    Illegal NheI site found at 1293
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3004
    Illegal BamHI site found at 1439
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1465
    Illegal NgoMIV site found at 1833
    Illegal NgoMIV site found at 1993
  • 1000
    COMPATIBLE WITH RFC[1000]