Difference between revisions of "Part:BBa K325903"

 
 
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<partinfo>BBa_K325903 short</partinfo>
 
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This part contains the Genes LuxE and LuxG from V.fischeri that have been codon optimised for use in E.coli. These genes are involved in the production of the substrate for the bacterial luciferase encoded by the LuxA and LuxB genes. As the proteins of the V.fischeri Lux operon are easily damaged by a high incubation temperature, transformed cells should not be grown at more than 30°C if light production is desired.
  
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=IISER_BHOPAL 2021=
 
===Usage and Biology===
 
===Usage and Biology===
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In P. fischeri, the first gene on the right operon, luxI is essential for forming autoinducer. Onn reaching threshold concentration, this autoinducer interacts with LuxR protein generation, a positive feedback loop that stimulates the transcription of the luxICDABEG.1. Reports showed that a hairpin loop structure has a poly(A) and poly(T) at the start and end respectively works as a bidirectional termination site for luxG.
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===References===
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Meighen, E. A. (1994). Genetics of Bacterial Bioluminescence. Annual Review of Genetics, 28(1), 117–139. https://doi.org/10.1146/annurev.ge.28.120194.001001
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Swartzman, A., Kapoor, S., Graham, A. F., & Meighen, E. A. (1990). A new Vibrio fischeri lux gene precedes a bidirectional termination site for the lux operon. Journal of Bacteriology, 172(12), 6797–6802. https://doi.org/10.1128/jb.172.12.6797-6802.1990
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K325903 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K325903 SequenceAndFeatures</partinfo>
  
  
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===Functional Parameters===
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==Functional Parameters: Austin_UTexas==
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<body>
 
<partinfo>BBa_K325903 parameters</partinfo>
 
<partinfo>BBa_K325903 parameters</partinfo>
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<h3><center>Burden Imposed by this Part:</center></h3>
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<figure>
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<div class = "center">
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<center><img src = "https://static.igem.org/mediawiki/parts/f/fa/T--Austin_Utexas--no_burden_icon.png" style = "width:160px;height:120px"></center>
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</div>
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<figcaption><center><b>Burden Value: 2.1 ± 6.2% </b></center></figcaption>
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<p> Burden is the percent reduction in the growth rate of <i>E. coli</i> cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the
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<a href="https://parts.igem.org/Part:BBa_K3174002">BBa_K3174002</a> - <a href="https://parts.igem.org/Part:BBa_K3174007">BBa_K3174007</a> pages for more information on the methods, an explanation of the sources of burden,  and other conclusions from a large-scale measurement project conducted by the <a href="http://2019.igem.org/Team:Austin_UTexas">2019 Austin_UTexas team</a>.</p>
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<p>This functional parameter was added by the <a href="https://2020.igem.org/Team:Austin_UTexas/Contribution">2020 Austin_UTexas team.</a></p>
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Latest revision as of 14:54, 9 October 2021

luxEG
V. Fischeri
(E. coli optimised)

This part contains the Genes LuxE and LuxG from V.fischeri that have been codon optimised for use in E.coli. These genes are involved in the production of the substrate for the bacterial luciferase encoded by the LuxA and LuxB genes. As the proteins of the V.fischeri Lux operon are easily damaged by a high incubation temperature, transformed cells should not be grown at more than 30°C if light production is desired.

IISER_BHOPAL 2021

Usage and Biology

In P. fischeri, the first gene on the right operon, luxI is essential for forming autoinducer. Onn reaching threshold concentration, this autoinducer interacts with LuxR protein generation, a positive feedback loop that stimulates the transcription of the luxICDABEG.1. Reports showed that a hairpin loop structure has a poly(A) and poly(T) at the start and end respectively works as a bidirectional termination site for luxG.

References

Meighen, E. A. (1994). Genetics of Bacterial Bioluminescence. Annual Review of Genetics, 28(1), 117–139. https://doi.org/10.1146/annurev.ge.28.120194.001001

Swartzman, A., Kapoor, S., Graham, A. F., & Meighen, E. A. (1990). A new Vibrio fischeri lux gene precedes a bidirectional termination site for the lux operon. Journal of Bacteriology, 172(12), 6797–6802. https://doi.org/10.1128/jb.172.12.6797-6802.1990


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 714
    Illegal AgeI site found at 929
    Illegal AgeI site found at 1517
  • 1000
    COMPATIBLE WITH RFC[1000]



Functional Parameters: Austin_UTexas

BBa_K325903 parameters

Burden Imposed by this Part:

Burden Value: 2.1 ± 6.2%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.