Difference between revisions of "Part:BBa K389013:Design"

(→‎Design Notes)
 
(3 intermediate revisions by the same user not shown)
Line 7: Line 7:
 
===Design Notes===
 
===Design Notes===
 
* medium strong constitutive promoter  
 
* medium strong constitutive promoter  
−
* double terminator (forward) to keep basal expression of mRFP low  
+
* The ''virG'' gene is mutated so it works without an ''rpoA'' subunit from ''Agrobacterium tumefaciens'' (YC Jung ''et al.'', 2004)
 +
* double terminator (forward) to keep expression of mRFP by the constitutive promoter low  
 
** double terminator <partinfo>BBa_B0017</partinfo> instead of <partinfo>BBa_B0015</partinfo> because of problems mentioned with <partinfo>BBa_B0012</partinfo>  
 
** double terminator <partinfo>BBa_B0017</partinfo> instead of <partinfo>BBa_B0015</partinfo> because of problems mentioned with <partinfo>BBa_B0012</partinfo>  
 
* mRFP gene to show the activity of the ''vir'' promoter
 
* mRFP gene to show the activity of the ''vir'' promoter
Line 13: Line 14:
 
===Source===
 
===Source===
  
−
parts.igem, synthetic gene by Mr. Gene, TI-plasmid from ''Agrobacterium tumefaciens'' C58
+
* ''virG'' gene synthesized by Mr. Gene (<partinfo>BBa_K389002</partinfo>)
 +
* ''vir'' promoter from ''A. tumefaciens'' C58 (<partinfo>BBa_K389003</partinfo>)
 +
* constitutive promoter, double terminator and mRFP from parts.igem (<partinfo>BBa_J23110</partinfo>, <partinfo>BBa_B0017</partinfo>, <partinfo>E1010</partinfo>)
  
 
===References===
 
===References===
 +
YC Jung ''et al.'' (2004) Mutants of ''Agrobacterium tumefaciens virG'' Gene That Activate Transcription of ''vir'' Promoter in ''Escherichia coli'', ''Current Microbiol'' 49:334-340.

Latest revision as of 15:10, 16 October 2010

VirA reporter system mRFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1686
    Illegal AgeI site found at 1798
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • medium strong constitutive promoter
  • The virG gene is mutated so it works without an rpoA subunit from Agrobacterium tumefaciens (YC Jung et al., 2004)
  • double terminator (forward) to keep expression of mRFP by the constitutive promoter low
  • mRFP gene to show the activity of the vir promoter

Source

References

YC Jung et al. (2004) Mutants of Agrobacterium tumefaciens virG Gene That Activate Transcription of vir Promoter in Escherichia coli, Current Microbiol 49:334-340.