Difference between revisions of "Part:BBa K389002:Design"

(→‎Design Notes)
(→‎Design Notes)
 
Line 10: Line 10:
 
* additional stop codon TAA added
 
* additional stop codon TAA added
 
* synthesized by Mr. Gene, so:  
 
* synthesized by Mr. Gene, so:  
−
** optimated codon usage for ''E. coli''  
+
** optimized codon usage for ''E. coli''  
 
** removal of illegal restriction sites
 
** removal of illegal restriction sites
  

Latest revision as of 14:49, 5 October 2010

Mutated virG


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • point mutations G56V and I77V so it works without rpoA gene from Agrobacterium tumefaciens in Escherichia coli (YC Jung et. al., 2004)
  • RBS (BBa_B0034-like) included
  • additional stop codon TAA added
  • synthesized by Mr. Gene, so:
    • optimized codon usage for E. coli
    • removal of illegal restriction sites

Source

synthetic

References

YC Jung et al. (2004) Mutants of Agrobacterium tumefaciens virG Gene That Activate Transcription of vir Promoter in Escherichia coli, Current Microbiol 49:334-340.