Difference between revisions of "Part:pSB1AC0"
(2 intermediate revisions by 2 users not shown) | |||
Line 2: | Line 2: | ||
<partinfo>pSB1AC0 short</partinfo> | <partinfo>pSB1AC0 short</partinfo> | ||
− | This part is derived from pSB1AC3-P1010. pSB1AC3-P1010 was cut with NotI (to remove the insert) and then religated together. Thus, pSB1AC0 is the empty plasmid in which the BioBricks cloning site has been partially destroyed. The EcoRI and PstI sites remain. | + | This part is derived from pSB1AC3-P1010. pSB1AC3-P1010 was cut with NotI (to remove the insert) and then religated together. Thus, pSB1AC0 is the empty plasmid in which the BioBricks cloning site has been partially destroyed. The EcoRI and PstI sites remain. It is a useful control in characterization experiments. This plasmid serves as a point of comparison to constructs of interest. Hence effects on morphology, fluorescence, growth rate etc. by the plasmid itself can be accounted for. |
+ | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
− | |||
+ | <!-- --> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>pSB1AC0 SequenceAndFeatures</partinfo> | <partinfo>pSB1AC0 SequenceAndFeatures</partinfo> | ||
+ | |||
+ | <!-- Uncomment this to enable Functional Parameter display | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>pSB1AC0 parameters</partinfo> | <partinfo>pSB1AC0 parameters</partinfo> | ||
+ | <!-- --> |
Latest revision as of 02:52, 5 June 2006
pSB1AC0
This part is derived from pSB1AC3-P1010. pSB1AC3-P1010 was cut with NotI (to remove the insert) and then religated together. Thus, pSB1AC0 is the empty plasmid in which the BioBricks cloning site has been partially destroyed. The EcoRI and PstI sites remain. It is a useful control in characterization experiments. This plasmid serves as a point of comparison to constructs of interest. Hence effects on morphology, fluorescence, growth rate etc. by the plasmid itself can be accounted for.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 3026
Illegal PstI site found at 8 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 3026
Illegal PstI site found at 8
Illegal NotI site found at 1 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 3026
Illegal XhoI site found at 1027
Illegal XhoI site found at 1919 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 3026
Illegal PstI site found at 8 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 3026
Illegal PstI site found at 8 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2065