Difference between revisions of "Part:BBa K389016"

 
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__NOTOC__
 
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<partinfo>BBa_K389016 short</partinfo>
 
<partinfo>BBa_K389016 short</partinfo>
  
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This BioBrick is for testing the virA/G signaling system. The read-out is the mRFP <partinfo>E1010</partinfo>. This system contains an unmutated ''virA'', so it is possible to measure, how the natural virA/G system works and reacts.
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This BioBrick contains a complete VirA/G receptor system with an mRFP (<partinfo>E1010</partinfo>) under the control of a ''vir'' promoter as reporter gene.  
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Input: acetosyringone
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Output: expression of [[Part:BBa_E1010 | mRFP]]
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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This BioBrick is for testing, characterizing and measuring the VirA/G signaling system. This system contains an unmutated ''virA'', so it is possible to measure how the natural VirA/G system works and reacts. It is also possible to determine the basal transcription of the ''vir'' promoter and its activity after inducing the system with acetosyringone.
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===Important parameters===
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<center>
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{|{{Table}}
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!Experiment
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!Characteristic
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!Value
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|-
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|rowspan="4"|[[Part:BBa_K389016:Experience#Transfer function of BBa_K389016 | Transfer function]]
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|Maximum induction level
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|2.6 fold
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|-
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|Maximum induction level reached
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|150 µM acetosyringone
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|-
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|Hill coefficient
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|1.67
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|-
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|[[Switch Point|Switch Point]]
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|26.5 µM acetosyringone
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|-
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|rowspan="4"|[[Part:BBa_K389016:Experience#Growth functions and mRFP expression for BBa_K389016 | Doubling time / h]]
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|without plasmid
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|1.98
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|-
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|carrying K389016
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|2.57
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|-
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|carrying K389016 with 150 µM acetosyringone
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|2.77
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|-
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|carrying K389016 with 1000 µM acetosyringone
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|3.01
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|-
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|rowspan="3"|[[Part:BBa_K389016:Experience#Plasmid conformation analysis | Conformation analysis]]
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|ratio ccc monomer / %
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|91.2
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|-
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|ratio ccc dimer / %
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|3.2
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|-
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|ratio oc forms / %
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|5.6
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|-
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|rowspan="5"|[[Part:BBa_K389016:Experience#Different possible inducers | Inducers]]
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|Induction by
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|Acetosyringone
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|-
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|rowspan="4"|No induction by
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|Capsaicin
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|-
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|Dopamine
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|-
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|Homovanillic acid
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|-
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|3-Methoxytyramine
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|}
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</center>
  
 
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Latest revision as of 00:17, 28 October 2010

VirA/G reporter device mRFP

This BioBrick contains a complete VirA/G receptor system with an mRFP (BBa_E1010) under the control of a vir promoter as reporter gene.

Input: acetosyringone

Output: expression of mRFP


Usage and Biology

This BioBrick is for testing, characterizing and measuring the VirA/G signaling system. This system contains an unmutated virA, so it is possible to measure how the natural VirA/G system works and reacts. It is also possible to determine the basal transcription of the vir promoter and its activity after inducing the system with acetosyringone.


Important parameters

Experiment Characteristic Value
Transfer function Maximum induction level 2.6 fold
Maximum induction level reached 150 µM acetosyringone
Hill coefficient 1.67
Switch Point 26.5 µM acetosyringone
Doubling time / h without plasmid 1.98
carrying K389016 2.57
carrying K389016 with 150 µM acetosyringone 2.77
carrying K389016 with 1000 µM acetosyringone 3.01
Conformation analysis ratio ccc monomer / % 91.2
ratio ccc dimer / % 3.2
ratio oc forms / % 5.6
Inducers Induction by Acetosyringone
No induction by Capsaicin
Dopamine
Homovanillic acid
3-Methoxytyramine

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 647
    Illegal NheI site found at 2581
    Illegal NheI site found at 2604
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1632
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 4260
    Illegal AgeI site found at 4372
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1768