Difference between revisions of "Part:BBa K389013:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K389013 short</partinfo>
 
<partinfo>BBa_K389013 short</partinfo>
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===Design Notes===
 
===Design Notes===
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medium strong constitutive promotor, terminator to keep basal expression of mRFP low
+
* medium strong constitutive promoter
−
 
+
* The ''virG'' gene is mutated so it works without an ''rpoA'' subunit from ''Agrobacterium tumefaciens'' (YC Jung ''et al.'', 2004)
−
 
+
* double terminator (forward) to keep expression of mRFP by the constitutive promoter low  
 +
** double terminator <partinfo>BBa_B0017</partinfo> instead of <partinfo>BBa_B0015</partinfo> because of problems mentioned with <partinfo>BBa_B0012</partinfo>
 +
* mRFP gene to show the activity of the ''vir'' promoter
  
 
===Source===
 
===Source===
  
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parts.igem, synthetic gene by Mr. Gene, TI-plasmid from ''Agrobacterium tumefaciens'' C58
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* ''virG'' gene synthesized by Mr. Gene (<partinfo>BBa_K389002</partinfo>)
 +
* ''vir'' promoter from ''A. tumefaciens'' C58 (<partinfo>BBa_K389003</partinfo>)
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* constitutive promoter, double terminator and mRFP from parts.igem (<partinfo>BBa_J23110</partinfo>, <partinfo>BBa_B0017</partinfo>, <partinfo>E1010</partinfo>)
  
 
===References===
 
===References===
 +
YC Jung ''et al.'' (2004) Mutants of ''Agrobacterium tumefaciens virG'' Gene That Activate Transcription of ''vir'' Promoter in ''Escherichia coli'', ''Current Microbiol'' 49:334-340.

Latest revision as of 15:10, 16 October 2010

VirA reporter system mRFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1686
    Illegal AgeI site found at 1798
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • medium strong constitutive promoter
  • The virG gene is mutated so it works without an rpoA subunit from Agrobacterium tumefaciens (YC Jung et al., 2004)
  • double terminator (forward) to keep expression of mRFP by the constitutive promoter low
  • mRFP gene to show the activity of the vir promoter

Source

References

YC Jung et al. (2004) Mutants of Agrobacterium tumefaciens virG Gene That Activate Transcription of vir Promoter in Escherichia coli, Current Microbiol 49:334-340.