Difference between revisions of "Part:BBa I50052"
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BBa_I50052 is a pSC101 temperature sensitive origin of replication. BBa_I50052 is the temperature sensitive replication origin found in the pSB8* series of BioBrick vectors as well as the pSIM5 recombineering vector. | BBa_I50052 is a pSC101 temperature sensitive origin of replication. BBa_I50052 is the temperature sensitive replication origin found in the pSB8* series of BioBrick vectors as well as the pSIM5 recombineering vector. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | This origin propagates at the permissive temperature of 30°C. At 37°C, the restrictive temperature, the repA protein becomes non-functional so that plasmids containing this origin can no longer propagate. It will take a few cell divisions for plasmid-free cells to arise and overtake the population. | ||
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Latest revision as of 18:25, 21 June 2010
pSC101 temperature sensitive origin of replication
BBa_I50052 is a pSC101 temperature sensitive origin of replication. BBa_I50052 is the temperature sensitive replication origin found in the pSB8* series of BioBrick vectors as well as the pSIM5 recombineering vector.
Usage and Biology
This origin propagates at the permissive temperature of 30°C. At 37°C, the restrictive temperature, the repA protein becomes non-functional so that plasmids containing this origin can no longer propagate. It will take a few cell divisions for plasmid-free cells to arise and overtake the population.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]