Difference between revisions of "Part:BBa K5198110"
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CTR-sfGFP is composed of the Human Trypsinogen-2 protein signal sequence (<a href="https://parts.igem.org/Part:BBa_K5198011">BBa_K5198011</a> | CTR-sfGFP is composed of the Human Trypsinogen-2 protein signal sequence (<a href="https://parts.igem.org/Part:BBa_K5198011">BBa_K5198011</a> | ||
) with sfGFP (<a href="https://parts.igem.org/Part:BBa_K5198012">BBa_K5198012</a> | ) with sfGFP (<a href="https://parts.igem.org/Part:BBa_K5198012">BBa_K5198012</a> | ||
| − | ), Superfolder green fluorescent protein. CTR is the proenzyme precursor to trypsin, and is secreted from the pancreas in this precursor form to later aid in digestion. At 51 bp long, the CTR signal sequence is relatively small and should not have significant effects on the protein attached | + | ), Superfolder green fluorescent protein. CTR is the proenzyme precursor to trypsin, and is secreted from the pancreas in this precursor form to later aid in digestion. At 51 bp long, the CTR signal sequence is relatively small and should not have significant effects on the protein attached. |
<div style="text-align: center;"> | <div style="text-align: center;"> | ||
Latest revision as of 12:04, 2 October 2024
CTR-sfGFP
CTR-sfGFP is composed of the Human Trypsinogen-2 protein signal sequence (BBa_K5198011
) with sfGFP (BBa_K5198012
), Superfolder green fluorescent protein. CTR is the proenzyme precursor to trypsin, and is secreted from the pancreas in this precursor form to later aid in digestion. At 51 bp long, the CTR signal sequence is relatively small and should not have significant effects on the protein attached.
Figure 2. Fluorescence readings of sfGFP with different signal peptide fusions in HEK293T cell supernatant. The negative control (NC) is sfGFP fused with an HA tag, which does not exhibit extracellular secretion. Error bars represent the standard deviation (SD) of 4 biological replicates. The data was normalized by subtracting background fluorescence.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 618
- 1000COMPATIBLE WITH RFC[1000]