Difference between revisions of "Part:BBa K5049005"

 
 
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Xylanases are enzymes that degrade xylan, a major component of plant cell walls, into simpler sugars. As feed additives, they play a crucial role in breaking down complex polysaccharides in animal diets, particularly for non-ruminants like poultry and swine. This enzymatic action enhances nutrient availability and digestion, leading to improved feed efficiency, growth performance, and overall health of the animals2. Moreover, the supplementation of xylanase in animal feed can significantly reduce feed costs and environmental impact by increasing nutrient absorption and decreasing nutrient excretion.
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Pir1, one of the Proteins with internal repeats (Pir) in Saccharomyces cerevisiae, another key anchor protein in addition to GPI, increases the choices for target proteins to be displayed. It is renowned for forming strong covalent bonds with cell wall components, enhancing enzyme durability under various conditions. This robust attachment keeps enzymes active and intact, supporting sustained industrial processes.   
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The sequence of DNA elements for xylanase was from Streptomyces thermovulgaris and Pir1 anchor protein was from Saccharomyces cerevisiae . This composite part was constructed by the basic parts of <a href="https://parts.igem.org/Part:BBa_K5049003">BBa_K5049003</a> and <a href="https://parts.igem.org/Part:BBa_K5049002">BBa_K5049002</a>
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Latest revision as of 04:05, 10 September 2024


Xylanase-Pir1

Xylanases are enzymes that degrade xylan, a major component of plant cell walls, into simpler sugars. As feed additives, they play a crucial role in breaking down complex polysaccharides in animal diets, particularly for non-ruminants like poultry and swine. This enzymatic action enhances nutrient availability and digestion, leading to improved feed efficiency, growth performance, and overall health of the animals2. Moreover, the supplementation of xylanase in animal feed can significantly reduce feed costs and environmental impact by increasing nutrient absorption and decreasing nutrient excretion.

Pir1, one of the Proteins with internal repeats (Pir) in Saccharomyces cerevisiae, another key anchor protein in addition to GPI, increases the choices for target proteins to be displayed. It is renowned for forming strong covalent bonds with cell wall components, enhancing enzyme durability under various conditions. This robust attachment keeps enzymes active and intact, supporting sustained industrial processes.

The sequence of DNA elements for xylanase was from Streptomyces thermovulgaris and Pir1 anchor protein was from Saccharomyces cerevisiae . This composite part was constructed by the basic parts of BBa_K5049003 and BBa_K5049002



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 529
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 856
    Illegal AgeI site found at 844
    Illegal AgeI site found at 1369
  • 1000
    COMPATIBLE WITH RFC[1000]