Difference between revisions of "Part:BBa K5198006"

 
 
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<partinfo>BBa_K5198006 short</partinfo>
 
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Minimal version of the Cytomegalovirus (CMV) promoter for expression in mammalian cells. The promoter can be used with upstream activating sequence in genetic designs for inducible expression.
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Minimal version of the Cytomegalovirus (CMV) promoter for expression in mammalian cells. miniCMV is a shorter version of the full-length CMV promoter that retains core elements of the CMV promoter for basic transcriptional activity.
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This variant of miniCMV was originally used as part of a Gal4UAS system (<a href="http://parts.igem.org/Part:BBa_K4839011">BBa_K4839011</a>), where it was placed downstream of upstream activating sequence (UAS) [1]. Upon binding of Gal4VP64 (<a href="http://parts.igem.org/Part:BBa_K3132000">BBa_K3132000</a>), the activity of miniCMV is enhanced, driving the expression of downstream genes.
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===Usage and Biology===
 
===Usage and Biology===
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<partinfo>BBa_K5198006 parameters</partinfo>
 
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===References===
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[1] L. Morsut *et al.*, “Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors,” *Cell*, vol. 164, no. 4, pp. 780–791, Feb. 2016, doi: [10.1016/j.cell.2016.01.012](https://doi.org/10.1016/j.cell.2016.01.012).

Latest revision as of 09:25, 2 October 2024


minimal CMV

Minimal version of the Cytomegalovirus (CMV) promoter for expression in mammalian cells. miniCMV is a shorter version of the full-length CMV promoter that retains core elements of the CMV promoter for basic transcriptional activity. This variant of miniCMV was originally used as part of a Gal4UAS system (BBa_K4839011), where it was placed downstream of upstream activating sequence (UAS) [1]. Upon binding of Gal4VP64 (BBa_K3132000), the activity of miniCMV is enhanced, driving the expression of downstream genes. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1] L. Morsut *et al.*, “Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors,” *Cell*, vol. 164, no. 4, pp. 780–791, Feb. 2016, doi: [10.1016/j.cell.2016.01.012](https://doi.org/10.1016/j.cell.2016.01.012).