Difference between revisions of "Part:BBa K200029"
 
(One intermediate revision by one other user not shown)
Line 3: Line 3:
  
 
This is the functional transcriptional unit which allows expression of the RcsB receiver protein and positive regulation of colanic acid production.  
 
This is the functional transcriptional unit which allows expression of the RcsB receiver protein and positive regulation of colanic acid production.  
 +
 +
The LacI promoter is normally inhibited by the Lac inhibitor, preventing any downstream translation. When IPTG is added, it binds to the Lac inhibitor, preventing inhibition of the promoter, allowing downstream expression of RcsB.
  
  
 
===Usage and Biology===
 
===Usage and Biology===
 
This composite part, comprising of subparts [https://parts.igem.org/Part:BBa_R0011 BBa_R0011], [https://parts.igem.org/Part:BBa_B0034 BBa_B0034], [https://parts.igem.org/Part:BBa_K200000 BBa_K200000] and [https://parts.igem.org/Part:BBa_B0015 BBa_B0015], was used in [http://2009.igem.org/Team:Imperial_College_London <i>The E.ncapsulator</i>] project by Imperial iGEM 2009.
 
This composite part, comprising of subparts [https://parts.igem.org/Part:BBa_R0011 BBa_R0011], [https://parts.igem.org/Part:BBa_B0034 BBa_B0034], [https://parts.igem.org/Part:BBa_K200000 BBa_K200000] and [https://parts.igem.org/Part:BBa_B0015 BBa_B0015], was used in [http://2009.igem.org/Team:Imperial_College_London <i>The E.ncapsulator</i>] project by Imperial iGEM 2009.
 +
 +
 +
===Characterization===
 +
Select the 'Experience' tab to see the characterization of this part by the UW-Madison 2010 Team.
 +
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 02:50, 28 October 2010

pLacI+RBS+RcsB+TT

This is the functional transcriptional unit which allows expression of the RcsB receiver protein and positive regulation of colanic acid production.

The LacI promoter is normally inhibited by the Lac inhibitor, preventing any downstream translation. When IPTG is added, it binds to the Lac inhibitor, preventing inhibition of the promoter, allowing downstream expression of RcsB.


Usage and Biology

This composite part, comprising of subparts BBa_R0011, BBa_B0034, BBa_K200000 and BBa_B0015, was used in [http://2009.igem.org/Team:Imperial_College_London The E.ncapsulator] project by Imperial iGEM 2009.


Characterization

Select the 'Experience' tab to see the characterization of this part by the UW-Madison 2010 Team.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]