Difference between revisions of "Part:BBa K4604013"

Latest revision as of 13:39, 11 October 2023

fluorescent protein mTurquoise

Fluorescent protein mTurquoise with an excitation maximum of 434 nm and emission maximum of 474 nm. Can be used together in a system with mVenus, another fluorescent protein, for FRET. This can be used to study protein-protein interactions. This BioBrick is mTurquoise with an N-terminal GS-linker to allow for fusion with another protein. In our case that was MazF, a toxin (BBa_K4604011). Also used and further characterized in BioBricks BBa_K4604017 and BBa_K4604018.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 43
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K4604013 short</partinfo>
-Fluorescent protein mTurquoise with an excitation maximum of 434 nm and emission maximum of 474 nm. Can be used together in a system with mVenus, another fluorescent protein, for FRET. This can be used to study protein-protein interactions.
+<html>
+Fluorescent protein mTurquoise with an excitation maximum of 434 nm and emission maximum of 474 nm. Can be used together in a system with mVenus, another fluorescent protein, for FRET. This can be used to study protein-protein interactions. This BioBrick is mTurquoise with an N-terminal GS-linker to allow for fusion with another protein. In our case that was MazF, a toxin (<a href="https://parts.igem.org/Part:BBa_K4604011">BBa_K4604011</a>). Also used and further characterized in BioBricks <a href="https://parts.igem.org/Part:BBa_K4604017">BBa_K4604017</a> and <a href="https://parts.igem.org/Part:BBa_K4604018">BBa_K4604018</a>.</html>
 <!-- Add more about the biology of this part here