Difference between revisions of "Part:BBa K4601100"
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<partinfo>BBa_K4601100 short</partinfo> | <partinfo>BBa_K4601100 short</partinfo> | ||
− | + | This part is a synthetic RBS specifically designed for the CatCh channelrhodopsin ([[Part:BBa_K4601000|BBa_K4601000]]) using the [https://www.denovodna.com/ Salis Lab RBS Library Calculator v2.0] [1-3]. | |
− | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
+ | This RBS was used to drive the expression of CatCh ([[Part:BBa_K4601000|BBa_K4601000]]) under the control of the T7 promoter ([[Part:BBa_K2150031|BBa_K2150031]]) in the composite part [[Part:BBa_K4601200|BBa_K4601200]]. | ||
+ | |||
+ | Using the Salis Lab RBS Library Calculator v2.0 [1-3], the predicted features of this RBS are: | ||
+ | Translation Initiation Rate (au) : 9603.17 | ||
+ | dG_total (kcal/mol) : -4.56 | ||
+ | dG_mRNA_rRNA (kcal/mol) : -10.65 | ||
+ | dG_spacing (kcal/mol) : 0 | ||
+ | dG_stacking (kcal/mol) : 0 | ||
+ | dG_standby (kcal/mol) : 1.09 | ||
+ | dG_start (kcal/mol) : -2.76 | ||
+ | dG_mRNA (kcal/mol) : -8.24 | ||
+ | Warnings : none | ||
+ | |||
+ | This RBS was selected as predicted to have a Translation Initiation Rate (TIR) of about 10000 au from a library of 128 RBSes (GGGTAGWGTATTCWMMTWAGGASGTAARTT) for which the estimated TIR range from 1424.62 to 1054132.03. | ||
+ | |||
+ | ===References=== | ||
+ | [1] Reis AC, Salis HM. An automated model test system for systematic development and improvement of gene expression models. ACS synthetic biology (2020) 9: 3145–3156. | ||
+ | |||
+ | [2] Farasat I, Kushwaha M, Collens J, Easterbrook M, Guido M, Salis HM. Efficient search, mapping, and optimization of multi-protein genetic systems in diverse bacteria. Molecular Systems Biology (2014) 10: 731. | ||
+ | |||
+ | [3] Ng CY, Farasat I, Maranas CD, Salis HM. Rational design of a synthetic Entner-Doudoroff pathway for improved and controllable NADPH regeneration. Metabolic Engineering (2015) 29: 86–96. | ||
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Latest revision as of 17:51, 2 October 2023
Synthetic RBS designed for CatCh
This part is a synthetic RBS specifically designed for the CatCh channelrhodopsin (BBa_K4601000) using the Salis Lab RBS Library Calculator v2.0 [1-3].
Usage and Biology
This RBS was used to drive the expression of CatCh (BBa_K4601000) under the control of the T7 promoter (BBa_K2150031) in the composite part BBa_K4601200.
Using the Salis Lab RBS Library Calculator v2.0 [1-3], the predicted features of this RBS are:
Translation Initiation Rate (au) : 9603.17 dG_total (kcal/mol) : -4.56 dG_mRNA_rRNA (kcal/mol) : -10.65 dG_spacing (kcal/mol) : 0 dG_stacking (kcal/mol) : 0 dG_standby (kcal/mol) : 1.09 dG_start (kcal/mol) : -2.76 dG_mRNA (kcal/mol) : -8.24 Warnings : none
This RBS was selected as predicted to have a Translation Initiation Rate (TIR) of about 10000 au from a library of 128 RBSes (GGGTAGWGTATTCWMMTWAGGASGTAARTT) for which the estimated TIR range from 1424.62 to 1054132.03.
References
[1] Reis AC, Salis HM. An automated model test system for systematic development and improvement of gene expression models. ACS synthetic biology (2020) 9: 3145–3156.
[2] Farasat I, Kushwaha M, Collens J, Easterbrook M, Guido M, Salis HM. Efficient search, mapping, and optimization of multi-protein genetic systems in diverse bacteria. Molecular Systems Biology (2014) 10: 731.
[3] Ng CY, Farasat I, Maranas CD, Salis HM. Rational design of a synthetic Entner-Doudoroff pathway for improved and controllable NADPH regeneration. Metabolic Engineering (2015) 29: 86–96.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]