Coding

Part:BBa_K4601000

Designed by: Doriane Blaise   Group: iGEM23_Evry-Paris-Saclay   (2023-09-23)


CatCh

This part is the E. coli codon optimised version of CatCh channelrhodopsin.

Usage and Biology

CatCh is a channelrhodopsin derived from the ChR2 of Chlamydomonas reinhardtii [1], from which it only retains the first 309 amino acids containing the N-terminal transmembrane domain forming. Compared to ChR2, it also contains one mutation L132C that modifies the photocurrent generated upon light irradiation. CatCh is a Ca2+ inward pump that is also capable of transporting, although to a lesser extent, Na+, K+ and Mg2+. It was successfully used in optogenetic therapies for visual restoration in primates [2].

For the characterisation of this part an expression cassette was assembled and the results are presented on its page in Parts Registry: BBa_K4601200.

References

[1] Kleinlogel S, Feldbauer K, Dempski RE, Fotis H, Wood PG, Bamann C, Bamberg E. Ultra light-sensitive and fast neuronal activation with the Ca2+-permeable channelrhodopsin CatCh. Nature Neuroscience (2011) 14: 513–518.

[2] Chaffiol A, Caplette R, Jaillard C, Brazhnikova E, Desrosiers M, Dubus E, Duhamel L, Macé E, Marre O, Benoit P, Hantraye P, Bemelmans A-P, Bamberg E, Duebel J, Sahel J-A, Picaud S, Dalkara D. A new promoter allows optogenetic vision restoration with enhanced sensitivity in macaque retina. Molecular Therapy: The Journal of the American Society of Gene Therapy (2017) 25: 2546–2560.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 623
    Illegal AgeI site found at 508
  • 1000
    COMPATIBLE WITH RFC[1000]


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