Difference between revisions of "Part:BBa K4390057"

Latest revision as of 23:38, 13 October 2022

His-tagged M. edulis MT expression construct

This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard which is also accepted by iGEM.

This is a level 1 part formed by assembly of the following level 0 parts:

Promoter	J23100
RBS	B0034
N part	K4390016
O part	K4390007
C part	K4390009
Terminator	K4390001

Usage and Biology

Metallothionein (MT) is a small protein (around 6-7 kDa) which is rich in cysteine. These thiol group in cysteines provide ability to chelate almost all heavy metal ions including Cd2+, Hg2+, Pb2+ and As3+, but had been shown that has higher binding affinity with Hg2+ (Manceau, A. et al., 2019). The ability of chelating heavy metals provides the metal tolerance for its hosts. This MT sequence was obtained from Mytilus edulis, a blue mussel which originally live in aqueous environment (MACKAY E. A. et al.,1993). This part is used to test the express M. edulis MT along with 6x-His tag for purification.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 11
Illegal NheI site found at 34
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 '''This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard [[Help:Standards/Assembly/Type_IIS|which is also accepted by iGEM.]]'''
+'''This is a level 1 part formed by assembly of the following level 0 parts:'''
+{| class="wikitable" style="margin:auto"
+|-
+| Promoter || [[part:BBa_J23100|J23100]]
+|-
+| RBS || [[part:BBa_B0034|B0034]]
+|-
+| N part || [[part:BBa_K4390016|K4390016]]
+|-
+| O part || [[part:BBa_K4390007|K4390007]]
+|-
+| C part || [[part:BBa_K4390009|K4390009]]
+|-
+| Terminator || [[part:BBa_K4390001|K4390001]]
+|}
 ==Usage and Biology==