Difference between revisions of "Part:BBa K4390057"
 
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<partinfo>BBa_K4390057 short</partinfo>
 
<partinfo>BBa_K4390057 short</partinfo>
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'''This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard [[Help:Standards/Assembly/Type_IIS|which is also accepted by iGEM.]]'''
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'''This is a level 1 part formed by assembly of the following level 0 parts:'''
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{| class="wikitable" style="margin:auto"
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|-
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| Promoter || [[part:BBa_J23100|J23100]]
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|-
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| RBS || [[part:BBa_B0034|B0034]]
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|-
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| N part || [[part:BBa_K4390016|K4390016]]
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|-
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| O part || [[part:BBa_K4390007|K4390007]]
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|-
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| C part || [[part:BBa_K4390009|K4390009]]
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|-
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| Terminator || [[part:BBa_K4390001|K4390001]]
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|}
  
 
==Usage and Biology==
 
==Usage and Biology==
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This part express  M. edulis MT along with 6x-His tag for purification.
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Metallothionein (MT) is a small protein (around 6-7 kDa) which is rich in cysteine. These thiol group in cysteines provide ability to chelate almost all heavy metal ions including Cd2+, Hg2+, Pb2+ and As3+, but had been shown that has higher binding affinity with Hg2+ (Manceau, A. et al., 2019). The ability of chelating heavy metals provides the metal tolerance for its hosts. This MT sequence was obtained from Mytilus edulis, a blue mussel which originally live in aqueous environment (MACKAY E. A. et al.,1993). This part is used to test the express  M. edulis MT along with 6x-His tag for purification.
  
 
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Latest revision as of 23:38, 13 October 2022


His-tagged M. edulis MT expression construct

This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard which is also accepted by iGEM.

This is a level 1 part formed by assembly of the following level 0 parts:

Promoter J23100
RBS B0034
N part K4390016
O part K4390007
C part K4390009
Terminator K4390001

Usage and Biology

Metallothionein (MT) is a small protein (around 6-7 kDa) which is rich in cysteine. These thiol group in cysteines provide ability to chelate almost all heavy metal ions including Cd2+, Hg2+, Pb2+ and As3+, but had been shown that has higher binding affinity with Hg2+ (Manceau, A. et al., 2019). The ability of chelating heavy metals provides the metal tolerance for its hosts. This MT sequence was obtained from Mytilus edulis, a blue mussel which originally live in aqueous environment (MACKAY E. A. et al.,1993). This part is used to test the express M. edulis MT along with 6x-His tag for purification.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]