Difference between revisions of "Part:BBa K4439012"

Latest revision as of 15:06, 12 October 2022

SR-AS-TEV-10xHis

Abstract

The SR-AS-TEV-10xHis protein was implemented in a pET28a vector. This protein could allow to test the polymerisation of [AS] modules (Part:BBa K3944000) present in recombinant green lacewing silk proteins N[AS]8C (Part:BBa K4439002) to create a very specific binding between two proteins.

No experiments were performed because of the time restriction, however one could do several interesting experiments combining this part with another of part containing recombinant green lacewing silk proteins (Part:BBa_K4439009).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 538
Illegal BglII site found at 607
Illegal BglII site found at 724
Illegal BglII site found at 920
Illegal BamHI site found at 757
Illegal XhoI site found at 924
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 The SR-AS-TEV-10xHis protein was implemented in a pET28a vector. This protein could allow to test the polymerisation of [AS] modules ([[Part:BBa K3944000]]) present in recombinant green lacewing silk proteins N[AS]8C ([[Part:BBa K4439002]]) to create a very specific binding between two proteins.
-No experiments were performed because of the time restriction, however one could do several interesting experiments combining this part with another of part containing recombinant green lacewing silk proteins ([[Part: BBa_K4439009]]).
+No experiments were performed because of the time restriction, however one could do several interesting experiments combining this part with another of part containing recombinant green lacewing silk proteins ([[Part:BBa_K4439009]]).
 ==Sequence and Features==
 <partinfo>BBa_K4439012 SequenceAndFeatures</partinfo>