Difference between revisions of "Part:BBa K4439012"
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==Abstract== | ==Abstract== | ||
| + | The SR-AS-TEV-10xHis protein was implemented in a pET28a vector. This protein could allow to test the polymerisation of [AS] modules ([[Part:BBa K3944000]]) present in recombinant green lacewing silk proteins N[AS]8C ([[Part:BBa K4439002]]) to create a very specific binding between two proteins. | ||
| + | No experiments were performed because of the time restriction, however one could do several interesting experiments combining this part with another of part containing recombinant green lacewing silk proteins ([[Part:BBa_K4439009]]). | ||
==Sequence and Features== | ==Sequence and Features== | ||
<partinfo>BBa_K4439012 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4439012 SequenceAndFeatures</partinfo> | ||
Latest revision as of 15:06, 12 October 2022
SR-AS-TEV-10xHis
Abstract
The SR-AS-TEV-10xHis protein was implemented in a pET28a vector. This protein could allow to test the polymerisation of [AS] modules (Part:BBa K3944000) present in recombinant green lacewing silk proteins N[AS]8C (Part:BBa K4439002) to create a very specific binding between two proteins.
No experiments were performed because of the time restriction, however one could do several interesting experiments combining this part with another of part containing recombinant green lacewing silk proteins (Part:BBa_K4439009).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 538
Illegal BglII site found at 607
Illegal BglII site found at 724
Illegal BglII site found at 920
Illegal BamHI site found at 757
Illegal XhoI site found at 924 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]