Difference between revisions of "Part:BBa K4368003"

Latest revision as of 18:52, 7 October 2022

pcstA + rbs + bglX + terminator

Description

BglX encodes for the β-glucosidase gene of Escherichia coli (BBa_K4368002). This enzyme is responsible of the degradation of cellulose working coordinated with the genes cenA and cex. In addition, this part includes the composition used by the team, which includes a strong rbs (BBa_B0030), a double terminator (BBa_B0015) as well as a promoter inducible by glucose concentration (BBa_K118011). The gene has been placed under the control of this promoter to build the glucose concentration-based gene regulatory circuit that integrates all our parts.

Characterization

The expression cassette sequence was digested with EcoRI and PstI enzymes and subsequently ligated with a chloramphenicol resistant plasmid backbone (Cm). Transforming bacteria were created with this plasmid and seeded on LB-Agar+Cm plates. After growth, colonies were selected based on their color (white) and DNA extraction was performed using the Promega PureYield Plasmid Miniprep System kit. The resulting DNA is used for further digestion with EcoRI and PstI. The digests are then run on a 0.75% agarose gel at 90 mV voltage and constant amperage. BioRad brand RedSafe is used as an intercalating developing agent.

Enzyme digestion

Sequence and Features

Contribution

• Group: UMA_MALAGA
• Author: Molina Calvo, Alonso