Difference between revisions of "Part:BBa K4115046"
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− | This Composite part is integrated into the plasmid backbone of PUC57 and entered Synechococcus HL7942 by natural transformation with E. coli. LuxR is a common receptor protein in bacterial quorum sensing. The LuxR in this composite part plays a role in receiving and responding to AHL(3O C6 HSL) signal molecules [1]. When LuxR receives the signal of AHL, it can regulate the downstream Lux pR [https://parts.igem.org/Part:BBa_R0062 (BBa_R0062)] to make superfolder GFP [https://parts.igem.org/Part:BBa_K4115000 (BBa_K4115000)] express.<br> | + | This Composite part is integrated into the plasmid backbone of PUC57 and entered <i>Synechococcus</i> HL7942 by natural transformation with <i>E. coli</i>. LuxR is a common receptor protein in bacterial quorum sensing. The LuxR in this composite part plays a role in receiving and responding to AHL(3O C6 HSL) signal molecules [1]. When LuxR receives the signal of AHL, it can regulate the downstream Lux pR [https://parts.igem.org/Part:BBa_R0062 (BBa_R0062)] to make superfolder GFP [https://parts.igem.org/Part:BBa_K4115000 (BBa_K4115000)] express.(More detailed infomation can be seen at the composite part [https://parts.igem.org/Part:BBa_K4115039 BBa_K4115039].)<br> |
We can construct a communication and regulation system between <i>E.coli</i> and <i>S.elongatus</i> HL7942<br> with Lux network. | We can construct a communication and regulation system between <i>E.coli</i> and <i>S.elongatus</i> HL7942<br> with Lux network. | ||
Latest revision as of 15:30, 11 October 2022
NS3-2-KanR-J23101-B0034-LuxR-B0015-Lux pR-B0034-sfGFP-B0015-NS3-1
NS3-2-lac UV5 promoter-cscB-lacI-KanR-NS3-1 | |
---|---|
Function | Gene regulator |
Use in | S.elongatus HL7942 |
RFC standard | None |
Backbone | pUC57 |
Submitted by | ShanghaiTech_China |
This Composite part is integrated into the plasmid backbone of PUC57 and entered Synechococcus HL7942 by natural transformation with E. coli. LuxR is a common receptor protein in bacterial quorum sensing. The LuxR in this composite part plays a role in receiving and responding to AHL(3O C6 HSL) signal molecules [1]. When LuxR receives the signal of AHL, it can regulate the downstream Lux pR (BBa_R0062) to make superfolder GFP (BBa_K4115000) express.(More detailed infomation can be seen at the composite part BBa_K4115039.)
We can construct a communication and regulation system between E.coli and S.elongatus HL7942
with Lux network.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 507
Illegal PstI site found at 4261 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1167
Illegal NheI site found at 1190
Illegal PstI site found at 507
Illegal PstI site found at 4261 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 507
Illegal PstI site found at 4261 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 507
Illegal PstI site found at 4261 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2145
References
[1] Kylilis, N., Tuza, Z.A., Stan, GB. et al. Tools for engineering coordinated system behaviour in synthetic microbial consortia. Nat Commun 9, 2677 (2018). https://doi.org/10.1038/s41467-018-05046-2