Difference between revisions of "Part:BBa T2018"
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<partinfo>BBa_T2018 short</partinfo> | <partinfo>BBa_T2018 short</partinfo> | ||
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This is a V5 epitope tag tail domain designed to be at the C-terminus of a protein. V5 tags are a type of epitope tag that can be used to measure the quantity of proteins. | This is a V5 epitope tag tail domain designed to be at the C-terminus of a protein. V5 tags are a type of epitope tag that can be used to measure the quantity of proteins. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | The V5 epitope tag is derived from amino acid residues 95-108, GKPIPNPLLGLDST, of RNA polymerase alpha subunit of simian virus 5. The V5 epitope tag is commonly added to proteins so that the tagged protein can be analyzed and visualized using immunochemical methods. Monoclonal and polyclonal antibodies to the V5 epitope tag are commercially available. | ||
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| + | The V5 epitope tag can be used on the N- or C-terminus of proteins. | ||
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Latest revision as of 14:54, 3 September 2009
V5 epitope tag tail domain (GKPIPNPLLGLDST)
This is a V5 epitope tag tail domain designed to be at the C-terminus of a protein. V5 tags are a type of epitope tag that can be used to measure the quantity of proteins.
Usage and Biology
The V5 epitope tag is derived from amino acid residues 95-108, GKPIPNPLLGLDST, of RNA polymerase alpha subunit of simian virus 5. The V5 epitope tag is commonly added to proteins so that the tagged protein can be analyzed and visualized using immunochemical methods. Monoclonal and polyclonal antibodies to the V5 epitope tag are commercially available.
The V5 epitope tag can be used on the N- or C-terminus of proteins.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]