Difference between revisions of "Part:BBa T2016:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_T2016 short</partinfo>
 
<partinfo>BBa_T2016 short</partinfo>
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===Design Notes===
 
===Design Notes===
−
This head domain begins with Met-Lys to promote efficient translation of the protein in ''E. coli''.
 
−
 
  
 +
This head domain begins with Met-Lys to promote efficient translation of the protein in ''E. coli''.  Rare codons from various model organisms were avoided where possible.
  
 
===Source===
 
===Source===
  
−
This is a synthetic sequence
+
This is a synthetic sequence.
  
 
===References===
 
===References===
 +
<biblio>
 +
#Kim93 pmid=8453373
 +
// appears to be original reference
 +
#Kim94 pmid=8059949
 +
#Fritze pmid=11044970
 +
</biblio>

Latest revision as of 13:12, 3 September 2009

S-tag head domain (KETAAAKFERQHMDS)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 16
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 10


Design Notes

This head domain begins with Met-Lys to promote efficient translation of the protein in E. coli. Rare codons from various model organisms were avoided where possible.

Source

This is a synthetic sequence.

References

<biblio>

  1. Kim93 pmid=8453373

// appears to be original reference

  1. Kim94 pmid=8059949
  2. Fritze pmid=11044970

</biblio>