Difference between revisions of "Part:BBa K4115046"
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K4115046 short</partinfo>
 
<partinfo>BBa_K4115046 short</partinfo>
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{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
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! colspan="2" style="background:#FFBF00;"|NS3-2-lac UV5 promoter-cscB-lacI-KanR-NS3-1
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|-
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|'''Function'''
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|Gene regulator
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|-
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|'''Use in'''
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|<i>S.elongatus</i> HL7942<br>
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|-
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|'''RFC standard'''
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|None<br>
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|-
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|'''Backbone'''
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|pUC57<br>
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|-
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|'''Submitted by'''
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|[https://2022.igem.wiki/shanghaitech-china/ ShanghaiTech_China]
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|}
  
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none
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This Composite part is integrated into the plasmid backbone of PUC57 and entered <i>Synechococcus</i> HL7942 by natural transformation with <i>E. coli</i>. LuxR is a common receptor protein in bacterial quorum sensing. The LuxR in this composite part plays a role in receiving and responding to AHL(3O C6 HSL) signal molecules [1]. When LuxR receives the signal of AHL, it can regulate the downstream Lux pR [https://parts.igem.org/Part:BBa_R0062 (BBa_R0062)] to make superfolder GFP [https://parts.igem.org/Part:BBa_K4115000 (BBa_K4115000)] express.(More detailed infomation can be seen at the composite part [https://parts.igem.org/Part:BBa_K4115039 BBa_K4115039].)<br>
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We can construct a communication and regulation system between <i>E.coli</i> and <i>S.elongatus</i> HL7942<br> with Lux network.
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This Composite part is integrated into the plasmid backbone of PUC57 and entered Synechococcus HL7942 by natural transformation with E. coli. LuxR is a common receptor protein in bacterial quorum sensing. The LuxR in this composite part plays a role in receiving and responding to AHL(3O C6 HSL) signal molecules, and sfGFP is designed here to detect the response intensity of LuxR. The higher the response intensity, the more sfGFP is expressed, and the more obvious the fluorescence is. Thus, we can construct a communicating system between E.coli and Synechococcus.
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<!-- Add more about the biology of this part here
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<partinfo>BBa_K4115046 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4115046 SequenceAndFeatures</partinfo>
  
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== References ==
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<small>
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[1] Kylilis, N., Tuza, Z.A., Stan, GB. et al. Tools for engineering coordinated system behaviour in synthetic microbial consortia. Nat Commun 9, 2677 (2018). https://doi.org/10.1038/s41467-018-05046-2
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 15:30, 11 October 2022


NS3-2-KanR-J23101-B0034-LuxR-B0015-Lux pR-B0034-sfGFP-B0015-NS3-1

NS3-2-lac UV5 promoter-cscB-lacI-KanR-NS3-1
Function Gene regulator
Use in S.elongatus HL7942
RFC standard None
Backbone pUC57
Submitted by ShanghaiTech_China

This Composite part is integrated into the plasmid backbone of PUC57 and entered Synechococcus HL7942 by natural transformation with E. coli. LuxR is a common receptor protein in bacterial quorum sensing. The LuxR in this composite part plays a role in receiving and responding to AHL(3O C6 HSL) signal molecules [1]. When LuxR receives the signal of AHL, it can regulate the downstream Lux pR (BBa_R0062) to make superfolder GFP (BBa_K4115000) express.(More detailed infomation can be seen at the composite part BBa_K4115039.)
We can construct a communication and regulation system between E.coli and S.elongatus HL7942
with Lux network.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 507
    Illegal PstI site found at 4261
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1167
    Illegal NheI site found at 1190
    Illegal PstI site found at 507
    Illegal PstI site found at 4261
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 507
    Illegal PstI site found at 4261
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 507
    Illegal PstI site found at 4261
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2145

References

[1] Kylilis, N., Tuza, Z.A., Stan, GB. et al. Tools for engineering coordinated system behaviour in synthetic microbial consortia. Nat Commun 9, 2677 (2018). https://doi.org/10.1038/s41467-018-05046-2