Difference between revisions of "Part:BBa K4491004"
 
(11 intermediate revisions by the same user not shown)
Line 8: Line 8:
 
| width=50% valign='top' style='border: 1px solid black'|
 
| width=50% valign='top' style='border: 1px solid black'|
 
<partinfo>BBa_K4491004 short</partinfo>
 
<partinfo>BBa_K4491004 short</partinfo>
−
* ultra strong double terminator characterised in the paper Precision design of stable genetic circuits carried in highly-insulated E. coli genomic landing pads (Park et al, 2018).  
+
* ultra strong double terminator characterised in the paper Precision design of stable genetic circuits carried in highly-insulated E. coli genomic landing pads (Park et al, 2018). :)
 +
 
  
 
* Double terminator allows transcription termination in both directions. This is important as during the codon optimisation process and when building genetic circuits, there is a chance that a reverse promoter can be produced somewhere along the genetic circuits.  
 
* Double terminator allows transcription termination in both directions. This is important as during the codon optimisation process and when building genetic circuits, there is a chance that a reverse promoter can be produced somewhere along the genetic circuits.  
  
 
Results characterised by Park et al., 2018
 
Results characterised by Park et al., 2018
−
Terminator strength (forward): 1800
+
* Terminator strength (forward): 1800
−
Terminator strength (reverse): 30
+
* Terminator strength (reverse): 30
  
 
|}
 
|}
 +
 +
 +
<html>
 +
</p>
 +
</html>
 +
__TOC__
 +
 +
{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
 +
! colspan="2" style="background:#FFBF00;"|DT54
 +
|-
 +
|'''Function'''
 +
|Termination
 +
|-
 +
|'''Use in'''
 +
|Bacteria
 +
|-
 +
|'''Chassis Tested'''
 +
|E. coli
 +
|-
 +
|'''Abstraction Hierarchy'''
 +
|Part
 +
|-
 +
|'''Related Device'''
 +
 +
|-
 +
|'''RFC standard'''
 +
|[https://parts.igem.org/Help:Assembly_standard_10 RFC10] & [https://parts.igem.org/Help:Assembly_standard_12 RFC12] & [https://parts.igem.org/Help:Assembly_standard_21 RFC21] & [https://parts.igem.org/Help:Assembly_standard_23 RFC23] & [https://parts.igem.org/Help:Assembly_standard_25 RFC25] & [https://parts.igem.org/Help:Assembly_standard_1000 RFC1000] compatible
 +
|-
 +
|'''Backbone'''
 +
|pJUMP18-Uac<br>
 +
|-
 +
|'''Submitted by'''
 +
|[https://2022.igem.wiki/cambridge/index.html Cambridge iGEM 2022]
 +
|}
 +
  
 
'''Secondary Structure'''
 
'''Secondary Structure'''
  
 
[[Image:Mfold-K4491004-1.png]]
 
[[Image:Mfold-K4491004-1.png]]
 +
 +
 +
 +
===Usage and Biology===
 +
 +
 +
===DNA Sequence===
  
 
<hr>
 
<hr>
 
'''Measurement'''
 
'''Measurement'''
 
* [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]
 
* [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]

Latest revision as of 13:57, 10 October 2022


Terminator.png

DT54

  • ultra strong double terminator characterised in the paper Precision design of stable genetic circuits carried in highly-insulated E. coli genomic landing pads (Park et al, 2018). :)


  • Double terminator allows transcription termination in both directions. This is important as during the codon optimisation process and when building genetic circuits, there is a chance that a reverse promoter can be produced somewhere along the genetic circuits.

Results characterised by Park et al., 2018

  • Terminator strength (forward): 1800
  • Terminator strength (reverse): 30


DT54
Function Termination
Use in Bacteria
Chassis Tested E. coli
Abstraction Hierarchy Part
Related Device
RFC standard RFC10 & RFC12 & RFC21 & RFC23 & RFC25 & RFC1000 compatible
Backbone pJUMP18-Uac
Submitted by Cambridge iGEM 2022


Secondary Structure

File:Mfold-K4491004-1.png


Usage and Biology

DNA Sequence

Measurement

  • [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]