Difference between revisions of "Part:BBa K190019:Design"
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K190019 short</partinfo>
 
<partinfo>BBa_K190019 short</partinfo>
−
fMT
 
−
 
−
fMT is a metallothionein, binding Arsenite(III) and Arsenate(V), it has higher affinity for As(III). Overexpression of fMT can be used to accumulate Arsenic without toxification of the cells.
 
  
 
<partinfo>BBa_K190019 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K190019 SequenceAndFeatures</partinfo>
Line 9: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
Add BioBrick prefix and suffix, also a RBS (B0034) will be added (not in sequence yet)
+
Add BioBrick prefix and suffix, also a RBS (<partinfo>B0034</partinfo>) will be added.<br>
 +
In working with heavy metal accumulation considerations should be made in the labwork required and also in the obtaining higher concentrations of the heavy metals in bacteria.
  
 
===Source===
 
===Source===
  
−
fMT was cloned from pMAL-MT (kindly provided by Wilfred Chen, University of California), the origin of the gene is the genome of ''Fucus vesiculosus''.
+
fMT was cloned from pMAL-MT (kindly provided by Wilfred Chen, University of California), the origin of the gene is the genome of ''Fucus vesiculosus''.<BR><BR>
 +
[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=nucleotide&dopt=GenBank&RID=AJPJ1GVY016&log%24=nuclalign&blast_rank=1&list_uids=3819155 GenBank: AJ005834.1]
  
 
===References===
 
===References===
−
Highly Selective and Rapid Arsenic Removal by Metabolically Engineered Escherichia coli Cells Expressing Fucus vesiculosus Metallothionein. Singh et al. App and Env Microbiology, May 2008, p. 2924–2927
+
 
 +
;Gold ''et al'', 2008
 +
:Gold B, Deng H, ''et al''(2008). "[http://www.ncbi.nlm.nih.gov/pubmed/18724363 Identification of a copper-binding metallothionein in pathogenic mycobacteria]". ''Nat Chem Biol.'' '''4(10)''': 609-616
 +
 
 +
;Klaassen ''et al''. 1994
 +
:Klaassen, Curtis D., Supratim Choudhuri, James M. McKim, Jr., Lois D. Lehman-McKeeman, and William C. Kershaw (1994). [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1567434/ "In Vitro and In Vivo Studies on the Degradation of Metallothionein"]. ''Environ. Health Perspect'' '''102(3)''': 141-146.
 +
 
 +
;Kostal 2004
 +
:Jan Kostal, Rosanna Yang, Cindy H. Wu, ''et al'' (August 2004). "[http://dx.doi.org/10.1128/AEM.70.8.4582-4587.2004 Enhanced Arsenic Accumulation in Engineered Bacterial Cells Expressing ArsR]". ''Applied and Environmental Microbiology'' 70(8): 4582–4587
 +
 
 +
;Morris ''et al'', 1999
 +
:C.A. Morris, B. Nicolaus, V. Sampson ''et al'' (March 1999). "[http://www.ncbi.nlm.nih.gov/pubmed/10024535 Identification and characterization of a recombinant metallothionein protein from a marine alga, <i>Fucus vesiculosus</i>]". '' The Biochemical journal'' '''338(2):''' 553-560
 +
 
 +
;Ngu ''et al.'' 2009
 +
:Thanh T. Ngu, J. A. Lee ''et al''. (April 2009). "[http://dx.doi.org/10.1021/bi9007462 Arsenic Metalation of Seaweed ''Fucus vesiculosus'' Metallothionein: The importance of the Interdomain Linker in Metallothionein]". ''Biochemistry'' '''48''': 8806–8816
 +
 
 +
;Singh ''et al'', 2008
 +
:Shailendra Singh, Ashok Mulchandani, Wilfred Chen (February 2008). "[http://dx.doi.org/10.1128/AEM.02871-07 Highly Selective and Rapid Arsenic Removal by Metabolically Engineered Escherichia coli Cells Expressing Fucus vesiculosus Metallothionein]". ''MICROBIOLOGY'' '''74(9)''': 2924–2927

Latest revision as of 22:32, 21 October 2009

fMT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 38
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 127
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Add BioBrick prefix and suffix, also a RBS (BBa_B0034) will be added.
In working with heavy metal accumulation considerations should be made in the labwork required and also in the obtaining higher concentrations of the heavy metals in bacteria.

Source

fMT was cloned from pMAL-MT (kindly provided by Wilfred Chen, University of California), the origin of the gene is the genome of Fucus vesiculosus.

[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=nucleotide&dopt=GenBank&RID=AJPJ1GVY016&log%24=nuclalign&blast_rank=1&list_uids=3819155 GenBank: AJ005834.1]

References

Gold et al, 2008
Gold B, Deng H, et al(2008). "[http://www.ncbi.nlm.nih.gov/pubmed/18724363 Identification of a copper-binding metallothionein in pathogenic mycobacteria]". Nat Chem Biol. 4(10): 609-616
Klaassen et al. 1994
Klaassen, Curtis D., Supratim Choudhuri, James M. McKim, Jr., Lois D. Lehman-McKeeman, and William C. Kershaw (1994). [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1567434/ "In Vitro and In Vivo Studies on the Degradation of Metallothionein"]. Environ. Health Perspect 102(3): 141-146.
Kostal 2004
Jan Kostal, Rosanna Yang, Cindy H. Wu, et al (August 2004). "[http://dx.doi.org/10.1128/AEM.70.8.4582-4587.2004 Enhanced Arsenic Accumulation in Engineered Bacterial Cells Expressing ArsR]". Applied and Environmental Microbiology 70(8): 4582–4587
Morris et al, 1999
C.A. Morris, B. Nicolaus, V. Sampson et al (March 1999). "[http://www.ncbi.nlm.nih.gov/pubmed/10024535 Identification and characterization of a recombinant metallothionein protein from a marine alga, Fucus vesiculosus]". The Biochemical journal 338(2): 553-560
Ngu et al. 2009
Thanh T. Ngu, J. A. Lee et al. (April 2009). "[http://dx.doi.org/10.1021/bi9007462 Arsenic Metalation of Seaweed Fucus vesiculosus Metallothionein: The importance of the Interdomain Linker in Metallothionein]". Biochemistry 48: 8806–8816
Singh et al, 2008
Shailendra Singh, Ashok Mulchandani, Wilfred Chen (February 2008). "[http://dx.doi.org/10.1128/AEM.02871-07 Highly Selective and Rapid Arsenic Removal by Metabolically Engineered Escherichia coli Cells Expressing Fucus vesiculosus Metallothionein]". MICROBIOLOGY 74(9): 2924–2927