Difference between revisions of "Part:BBa K4439012"

 
 
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<partinfo>BBa_K4439012 short</partinfo>
 
<partinfo>BBa_K4439012 short</partinfo>
  
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SR-AS-TEV-10xHis  
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==Abstract==
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The SR-AS-TEV-10xHis protein was implemented in a pET28a vector. This protein could allow to test the polymerisation of [AS] modules ([[Part:BBa K3944000]]) present in recombinant green lacewing silk proteins N[AS]8C ([[Part:BBa K4439002]]) to create a very specific binding between two proteins.
  
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No experiments were performed because of the time restriction, however one could do several interesting experiments combining this part with another of part containing recombinant green lacewing silk proteins ([[Part:BBa_K4439009]]).
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===Usage and Biology===
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==Sequence and Features==
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4439012 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4439012 SequenceAndFeatures</partinfo>
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===Functional Parameters===
 
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<partinfo>BBa_K4439012 parameters</partinfo>
 
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Latest revision as of 15:06, 12 October 2022

SR-AS-TEV-10xHis

Abstract

The SR-AS-TEV-10xHis protein was implemented in a pET28a vector. This protein could allow to test the polymerisation of [AS] modules (Part:BBa K3944000) present in recombinant green lacewing silk proteins N[AS]8C (Part:BBa K4439002) to create a very specific binding between two proteins.

No experiments were performed because of the time restriction, however one could do several interesting experiments combining this part with another of part containing recombinant green lacewing silk proteins (Part:BBa_K4439009).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 538
    Illegal BglII site found at 607
    Illegal BglII site found at 724
    Illegal BglII site found at 920
    Illegal BamHI site found at 757
    Illegal XhoI site found at 924
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]