Difference between revisions of "Part:BBa K4226002"
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<partinfo>BBa_K4226002 short</partinfo> | <partinfo>BBa_K4226002 short</partinfo> | ||
| − | This part is an improvement for the part：[[BBa_K1014000|HrpS Gene]] uploaded by iGEM13_HIT-Harbin. The hrpS Gene codes for hrpS protein. The hrpR protein binds to hrpS protein forming a complex, causing a transcriptionally upregulated of | + | This part is an improvement for the part：[[BBa_K1014000|HrpS Gene]] uploaded by iGEM13_HIT-Harbin. The hrpS Gene codes for hrpS protein. The hrpR protein binds to hrpS protein forming a complex, causing a transcriptionally upregulated of [[Part:BBa K4226003|hrpL Promoter]]. The [[Part:BBa_K4226004|Amp30E Amplification Device]] consists of [[Part:BBa K4226001|hrpR Gene]], hrpS and [[Part:BBa K4226003|hrpL Promoter]]. |
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Latest revision as of 09:59, 11 October 2022
hrpS Gene
This part is an improvement for the part：HrpS Gene uploaded by iGEM13_HIT-Harbin. The hrpS Gene codes for hrpS protein. The hrpR protein binds to hrpS protein forming a complex, causing a transcriptionally upregulated of hrpL Promoter. The Amp30E Amplification Device consists of hrpR Gene, hrpS and hrpL Promoter.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 902
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 112
Illegal SapI.rc site found at 745
Reference
[1] Wan, Xinyi, et al. "Cascaded amplifying circuits enable ultrasensitive cellular sensors for toxic metals." Nature chemical biology 15.5 (2019): 540-548.