Difference between revisions of "Part:BBa K4226002"
 
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<partinfo>BBa_K4226002 short</partinfo>
 
<partinfo>BBa_K4226002 short</partinfo>
  
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This part is an improvement for the part：[[BBa_K1014000|HrpS Gene]] uploaded by iGEM13_HIT-Harbin. The hrpS Gene codes for hrpS protein. The hrpR protein binds to hrpS protein forming a complex, causing a transcriptionally upregulated of hrpL promoter ([[Part:BBa K4226003|hrpL Promoter]]) . The Amp30E Amplification Device consists of hrpR ([[Part:BBa K4226001|hrpR Gene]]), hrpS and hrpL promoter ([[Part:BBa K4226003|hrpL Promoter]]) .
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This part is an improvement for the part：[[BBa_K1014000|HrpS Gene]] uploaded by iGEM13_HIT-Harbin. The hrpS Gene codes for hrpS protein. The hrpR protein binds to hrpS protein forming a complex, causing a transcriptionally upregulated of [[Part:BBa K4226003|hrpL Promoter]]. The [[Part:BBa_K4226004|Amp30E Amplification Device]] consists of [[Part:BBa K4226001|hrpR Gene]], hrpS and [[Part:BBa K4226003|hrpL Promoter]].
  
 
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<partinfo>BBa_K4226002 parameters</partinfo>
 
<partinfo>BBa_K4226002 parameters</partinfo>
 
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<b>Reference</b>
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<br>
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[1] Wan, Xinyi, et al. "Cascaded amplifying circuits enable ultrasensitive cellular sensors for toxic metals." Nature chemical biology 15.5 (2019): 540-548.<br>

Latest revision as of 09:59, 11 October 2022


hrpS Gene

This part is an improvement for the part：HrpS Gene uploaded by iGEM13_HIT-Harbin. The hrpS Gene codes for hrpS protein. The hrpR protein binds to hrpS protein forming a complex, causing a transcriptionally upregulated of hrpL Promoter. The Amp30E Amplification Device consists of hrpR Gene, hrpS and hrpL Promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 902
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 112
    Illegal SapI.rc site found at 745


Reference
[1] Wan, Xinyi, et al. "Cascaded amplifying circuits enable ultrasensitive cellular sensors for toxic metals." Nature chemical biology 15.5 (2019): 540-548.