Difference between revisions of "Part:BBa K4197019"
 
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<partinfo>BBa_K4197019 short</partinfo>
 
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Gene coding for DARPin e2_79 which is a protein that recognises the constant part of IgE.  
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Gene coding for DARPin e2_79 which is a protein that recognises the constant part of IgE (Baumann et al., 2010).  
  
  
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<h2>Introduction</h2>
 
<h2>Introduction</h2>
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<p>This part is composed of the gene coding for the DARPin E2_79 protein. DARPin is a protein that recognizes the constant part of IgE (Baumann et al., 2010). It has already been expressed in <i> E. coli </i> </p>
  
 
<h2>Construction</h2>
 
<h2>Construction</h2>
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<p>The objective of the INSA-UPS 2022 team was to display DARPin E2_79 on the surface of <i>E. coli</i> so the ordered sequence was merged to OmpA membrane lipoprotein (see part <a href="https://parts.igem.org/Part:BBa_K4197011">BBa_K1694011</a>).</p>
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                    <b>Figure 1: </b> <b>Xxxxxx</b> 
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                <b>Figure 2: </b> <b>Xxxxxxxxxxxxx</b> 
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<h2>titre 2</h2>
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<h3>Titre 3</h3>
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    <li>Forward : TAAGAAGGAGATATACCATGGCGGAAGCGGGTATCACC</li>
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    <li>Reverse : CTCGAGTGCGGCCGCAAGCTTCGGATCGTCCTATGATGGAGG</li>
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    <li>CForward : CGCGGCCGCTTCTAGAGCGGAAGCGGGTATCACC</li>
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    <li>Reverse : AGCGGCCGCTACTAGTCGGATCGTCCTATGATGGAGG</li>
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<h2>References</h2>
  
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<h3>titre 3</h3>
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<p>More information about the project for which the part was created:<a href="https://2022.igem.wiki/toulouse-insa-ups/index.html"> DAISY (INSA-UPS 2022)</a> </p>
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    <h4>Titre 4</h4>
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<p>Xxxxxx</p>
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<p>Other parts to display allergens:<br>
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<h4>Titre 4</h4>
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  <p>Other parts using the DARPin:<br>
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<p>xxxxxxx</p>
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<h2>Titre 2</h2>
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- <a href="https://parts.igem.org/Part:BBa_K4197011">OmpA_DARPin</a><br>
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<p>Xxxxxx</p>
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- <a href="https://parts.igem.org/Part:BBa_K4197010">OmpA_DARPin_sfGFP</a><br>
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<h2>References</h2>
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- <a href="https://parts.igem.org/Part:BBa_K4197001"> OmpA_DARPin fusion + ihfB800-mTagBFP</a><br>
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</p>
 
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     <i>
 
     <i>
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     <li>Morag E, Lapidot A, Govorko D, Lamed R, Wilchek M, Bayer EA, Shoham Y: Expression, purification, and characterization of the cellulose-binding domain of the scaffoldin subunit from the cellulosome of Clostridium thermocellum. Applied and Environmental Microbiology 1995, 61:1980-1986.</li>
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     <li>Baumann, M. J., Eggel, A., Amstutz, P., Stadler, B. M., & Vogel, M. (2010). DARPins against a functional IgE epitope. Immunology Letters, 133(2), 78–84. https://doi.org/10.1016/j.imlet.2010.07.005 </li>
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    <li>Nogueira ES, Schleier T, Durrenberger M, Ballmer-Hofer K, Ward TR, Jaussi R: High-level secretion of recombinant full-length streptavidin in Pichia pastoris and its application to enantioselective catalysis. Protein Expr Purif 2014, 93:54-62. DOI: 10.1016/j.pep.2013.10.015.</li>
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    <li>Young TS, Schultz PG: Beyond the canonical 20 amino acids: expanding the genetic lexicon. J Biol Chem 2010, 285:11039-11044. DOI: 10.1074/jbc.R109.091306.</li>
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</i>
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4197014 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K4197019 SequenceAndFeatures</partinfo>
  
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K4197014 parameters</partinfo>
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<partinfo>BBa_K4197019 parameters</partinfo>
 
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Latest revision as of 22:29, 8 October 2022


DARPin e2_79

Gene coding for DARPin e2_79 which is a protein that recognises the constant part of IgE (Baumann et al., 2010).


Introduction

This part is composed of the gene coding for the DARPin E2_79 protein. DARPin is a protein that recognizes the constant part of IgE (Baumann et al., 2010). It has already been expressed in E. coli

Construction

The objective of the INSA-UPS 2022 team was to display DARPin E2_79 on the surface of E. coli so the ordered sequence was merged to OmpA membrane lipoprotein (see part BBa_K1694011).

References

More information about the project for which the part was created: DAISY (INSA-UPS 2022)

Other parts to display allergens:

Other parts using the DARPin:
- OmpA_DARPin
- OmpA_DARPin_sfGFP
- OmpA_DARPin fusion + ihfB800-mTagBFP

  1. Baumann, M. J., Eggel, A., Amstutz, P., Stadler, B. M., & Vogel, M. (2010). DARPins against a functional IgE epitope. Immunology Letters, 133(2), 78–84. https://doi.org/10.1016/j.imlet.2010.07.005


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 205
  • 1000
    COMPATIBLE WITH RFC[1000]