Difference between revisions of "Part:BBa K4060100:Experience"

Latest revision as of 13:57, 7 December 2021

1. Assembly of Pre A1 (pSB1A2)

The gene fragment Pre A1 is composed of BBa_K206000) and BBa_B0034. We digested the vector fragment BBa_B0034 with XbaI and EcoRI and the insert fragment BBa_K206000 digested with EcoRI and SpeI. We ligated these fragments together to form Pre A1.

Fig. 1 Agarose electrophoresis in order to verify Pre A1. On the right is the expected results and on the left is the obtained confirmation agarose gel. Lane 1, 100bp ladder. Lane 1’, partial of Life 1 kb Plus ladder. Lane 2, Pre A1 digested with BamHI EcoRI PstI. Lane 3, Pre A1 digested with NheI EcoRI PstI. Lane 4, Pre A1 digested with EcoRI PstI.

Applications of BBa_K4060100

User Reviews

UNIQf7b65e303044c661-partinfo-00000005-QINU UNIQf7b65e303044c661-partinfo-00000006-QINU

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 __NOTOC__
-This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
+====1. Assembly of Pre A1 (pSB1A2)====
-how you used this part and how it worked out.
+The gene fragment Pre A1 is composed of <partinfo>BBa_K206000</partinfo>) and <partinfo>BBa_B0034</partinfo>. We digested the vector fragment <partinfo>BBa_B0034</partinfo> with XbaI and EcoRI and the insert fragment <partinfo>BBa_K206000</partinfo> digested with EcoRI and SpeI. We ligated these fragments together to form Pre A1.
+<html><body>
+<img src="https://2021.igem.org/wiki/images/e/eb/T--NYCU-Taipei--_4A5AFBB2-B701-471F-B773-F9C10E018FAA_4_5005_c.jpeg" height="400px" width="750px"/>
+</body></html>
+Fig. 1 Agarose electrophoresis in order to verify Pre A1. On the right is the expected results and on the left is the obtained confirmation agarose gel. Lane 1, 100bp ladder. Lane 1’, partial of Life 1 kb Plus ladder. Lane 2, Pre A1 digested with BamHI EcoRI PstI. Lane 3, Pre A1 digested with NheI EcoRI PstI. Lane 4, Pre A1 digested with EcoRI PstI.
 ===Applications of BBa_K4060100===