Difference between revisions of "Part:BBa K3861004"
 
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lldR coding sequence of <i>Salmonella</i> Typhimurium ATCC14028 which is part of the lactate inducer system. lldR is responsible for the induction of the lldRPD operon and encodes a putative regulatory protein.<sup>1</sup> Further, the lldR operon induces the lactate metabolism if lactate is the sole carbon source (). Part of the <a href="https://parts.igem.org/Part:BBa_K3861005">BBa_K3861005</a> backbone.  
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<i>lldR</i> coding sequence from <i>Salmonella</i> Typhimurium ATCC14028. LldR regulates the transcription of the <I>lldPRD</i> operon which is involved in lactate metabolism. LldR represses the expression of the the <i>lldRPD</i> operon genes by binding to two operator regions of PlldR promoter and forming a loop.<sup>1,2</sup>. The lldR gene will be constituvely expressed in our designed backbone pKF01 (<a href="https://parts.igem.org/Part:BBa_K3861005">BBa_K3861005</a>), and will allow for tight regulation of genes under the lldR promoter (<a href="https://parts.igem.org/Part:BBa_K1847008">BBa_K1847008</a>).
 
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==='''References'''===
 
==='''References'''===
 
1. Gillis, C. C. et al. Host-Derived Metabolites Modulate Transcription of Salmonella Genes Involved in l-Lactate Utilization during Gut Colonization. <i>Infect. Immun. 87</i>, e00773-18 (2019).
 
1. Gillis, C. C. et al. Host-Derived Metabolites Modulate Transcription of Salmonella Genes Involved in l-Lactate Utilization during Gut Colonization. <i>Infect. Immun. 87</i>, e00773-18 (2019).
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2. 1. Aguilera, L. et al. Dual role of LldR in regulation of the lldPRD operon, involved in L-lactate metabolism in Escherichia coli. J. Bacteriol. (2008) doi:10.1128/JB.02013-07.

Latest revision as of 22:30, 21 October 2021


lldR

lldR coding sequence from Salmonella Typhimurium ATCC14028. LldR regulates the transcription of the lldPRD operon which is involved in lactate metabolism. LldR represses the expression of the the lldRPD operon genes by binding to two operator regions of PlldR promoter and forming a loop.1,2. The lldR gene will be constituvely expressed in our designed backbone pKF01 (BBa_K3861005), and will allow for tight regulation of genes under the lldR promoter (BBa_K1847008).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1. Gillis, C. C. et al. Host-Derived Metabolites Modulate Transcription of Salmonella Genes Involved in l-Lactate Utilization during Gut Colonization. Infect. Immun. 87, e00773-18 (2019). 2. 1. Aguilera, L. et al. Dual role of LldR in regulation of the lldPRD operon, involved in L-lactate metabolism in Escherichia coli. J. Bacteriol. (2008) doi:10.1128/JB.02013-07.