Difference between revisions of "Part:BBa K3889051"
 
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In addition to PhoD (BBa_K3889050) another known Twin Arginine Translocase(Tat) signal peptide is the leader sequence of Bacillus subtilis substrate YwbN. However, unlike PhoD signal peptide, this signal peptide directs the protein through the Tat secretory pathway exclusively.
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In addition to PhoD (<partinfo>BBa_K3889050</partinfo>) another known Twin Arginine Translocase(Tat) signal peptide is the leader sequence of Bacillus subtilis substrate YwbN. However, unlike PhoD signal peptide, this signal peptide directs the protein through the Tat secretory pathway exclusively.
  
 
Although the efficiency of secretion is ultimately dependent on multiple factors including conformation of the protein, cellular environment, etc. this part might be used for secretion of different recombinant proteins in Bacillus subtilis
 
Although the efficiency of secretion is ultimately dependent on multiple factors including conformation of the protein, cellular environment, etc. this part might be used for secretion of different recombinant proteins in Bacillus subtilis

Latest revision as of 17:52, 18 October 2021


Bacillus subtilis TAT signal peptide YwbN


In addition to PhoD (BBa_K3889050) another known Twin Arginine Translocase(Tat) signal peptide is the leader sequence of Bacillus subtilis substrate YwbN. However, unlike PhoD signal peptide, this signal peptide directs the protein through the Tat secretory pathway exclusively.

Although the efficiency of secretion is ultimately dependent on multiple factors including conformation of the protein, cellular environment, etc. this part might be used for secretion of different recombinant proteins in Bacillus subtilis


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



References

1. Kolkman, M. A., van der Ploeg, R., Bertels, M., van Dijk, M., van der Laan, J., van Dijl, J. M., & Ferrari, E. (2008). The twin-arginine signal peptide of Bacillus subtilis YwbN can direct either Tat- or Sec-dependent secretion of different cargo proteins: secretion of active subtilisin via the B. subtilis Tat pathway. Applied and environmental microbiology, 74(24), 7507–7513. https://doi.org/10.1128/AEM.01401-08

2. Peng, C., Shi, C., Cao, X., Li, Y., Liu, F., & Lu, F. (2019). Factors Influencing Recombinant Protein Secretion Efficiency in Gram-Positive Bacteria: Signal Peptide and Beyond. Frontiers in bioengineering and biotechnology, 7, 139. https://doi.org/10.3389/fbioe.2019.00139

3. Frain, K. M., van Dijl, J. M., & Robinson, C. (2019). The Twin-Arginine Pathway for Protein Secretion. EcoSal Plus, 8(2), 10.1128/ecosalplus.ESP-0040-2018. https://doi.org/10.1128/ecosalplus.ESP-0040-2018