Difference between revisions of "Part:BBa K3930006"
 
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<h2>Introduction</h2>
 
<h2>Introduction</h2>
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<p>The Z3eV assembly  is composed of the promoter TEF1, the gene <i>Z3eV</i> coding for the activator Z3eV of the estradiol inducible promoter BBa_K3930007 and the transcription terminator of CYC1. The construction is based on the publication (Liu et al. 2020). </p>
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<p>The Z3eV assembly  is composed of the promoter TEF1, the gene <i>Z3eV</i> coding for the activator Z3eV of the estradiol inducible promoter BBa_K3930007 and the transcription terminator of CYC1. The construction is based on the publication (Liu et al., 2020). </p>
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<p>This sequence must be used with the promoter Z3eV (BBa_K3930007), for a gene regulated expression into <i>S.cerevisiae</i>.  
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<p>This sequence must be used with the promoter Z3eV <a href="https://parts.igem.org/Part:BBa_K3930007" class="pr-0" target="_blank">(BBa_K3930007)</a>, for a gene regulated expression into <i>S. cerevisiae</i>.  
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<h2>Testing</h2>
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<p>This part is one of the component of the pFLEUR part <a href="https://parts.igem.org/Part:BBa_K3930000" class="pr-0" target="_blank">(BBa_K3930000)</a> and has been succesfully cloned into the LycoYeast <i>S. cerevisiae</i> strain genome. The Toulouse team could not test this part for reasons of time, and preferred to focus on the part characterizations that allow the production of &alpha; and &beta;-ionone for the floral aspect of the violet perfume.</p>
 
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<h2>References</h2>
 
<h2>References</h2>

Latest revision as of 17:04, 16 October 2021


Inducer Z3EV of the estradiol promoter Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1021
    Illegal XbaI site found at 1324
    Illegal PstI site found at 666
    Illegal PstI site found at 837
    Illegal PstI site found at 1340
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 666
    Illegal PstI site found at 837
    Illegal PstI site found at 1340
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1021
    Illegal XbaI site found at 1324
    Illegal PstI site found at 666
    Illegal PstI site found at 837
    Illegal PstI site found at 1340
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1021
    Illegal XbaI site found at 1324
    Illegal PstI site found at 666
    Illegal PstI site found at 837
    Illegal PstI site found at 1340
    Illegal AgeI site found at 1527
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1849
    Illegal BsaI.rc site found at 987

Introduction

The Z3eV assembly is composed of the promoter TEF1, the gene Z3eV coding for the activator Z3eV of the estradiol inducible promoter BBa_K3930007 and the transcription terminator of CYC1. The construction is based on the publication (Liu et al., 2020).

This sequence must be used with the promoter Z3eV (BBa_K3930007), for a gene regulated expression into S. cerevisiae.

Testing

This part is one of the component of the pFLEUR part (BBa_K3930000) and has been succesfully cloned into the LycoYeast S. cerevisiae strain genome. The Toulouse team could not test this part for reasons of time, and preferred to focus on the part characterizations that allow the production of α and β-ionone for the floral aspect of the violet perfume.


References

  1. Liu J, Wang T, Jiang Y, Liu Z, Tian P, Wang F, Deng L. 2020. Harnessing β-estradiol inducible expression system to overproduce nervonic acid in Saccharomyces cerevisiae. Process Biochemistry. 92:37–42. doi:10.1016/j.procbio.2020.02.032.