Difference between revisions of "Part:pSB1AT3"

(→‎Usage and Biology)
(→‎Usage and Biology)
 
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Primers for these Biobrick vectors can be found in [[Part:BBa_G00100]] (aka VF2) and [[Part:BBa_G00101]] (aka. VR). See also [[Part:BBa_J18907]] (aka VR2) for lower temperature sequencing reactions.
 
Primers for these Biobrick vectors can be found in [[Part:BBa_G00100]] (aka VF2) and [[Part:BBa_G00101]] (aka. VR). See also [[Part:BBa_J18907]] (aka VR2) for lower temperature sequencing reactions.
  
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*This vector is also available in the Fusion (Freiburg) Biobrick format: [[Part:BBa_J18903]]  
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*This vector is also available in the Fusion (Freiburg) Biobrick format: [[Part:BBa_J18903]] (pSB1AT3F)
  
  

Latest revision as of 15:54, 26 February 2009

High copy BioBrick assembly plasmid

pSB1AT3 is a high copy number plasmid carrying ampicillin and tetracycline resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).

Annotated [http://www.biology.utah.edu/jorgensen/wayned/ape/ APE] file for pSB1AT3
Plasmid map for pSB1AT3

Usage and Biology

Tetracycline resistant up to 150 ug/ml.

Kanamycin sensitive at 5 ug/ml Chloramphicol sensitive at 3.5 ug/ml (spayne and tk 6/6/05)

Primers for these Biobrick vectors can be found in Part:BBa_G00100 (aka VF2) and Part:BBa_G00101 (aka. VR). See also Part:BBa_J18907 (aka VR2) for lower temperature sequencing reactions.

  • This vector is also available in the Fusion (Freiburg) Biobrick format: Part:BBa_J18903 (pSB1AT3F)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3425
    Illegal NheI site found at 1270
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 3431
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3425
    Illegal BamHI site found at 1416
    Illegal XhoI site found at 1035
    Illegal XhoI site found at 2318
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3425
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3425
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3440
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 1442
    Illegal NgoMIV site found at 1810
    Illegal NgoMIV site found at 1970
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2464